Invitrogen

Phospho-STAT5 (Tyr694) Monoclonal Antibody (SRBCZX), PE, eBioscience™

Phospho-STAT5 (Tyr694) Antibody in Flow Cytometry (Flow)

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Phospho-STAT5 (Tyr694) Antibody (12-9010-42) in Flow

LEFT: Intracellular staining of untreated (orange histogram) or 15-minute IL-2-treated (purple histogram) human Th2-polarized CD4+ with Anti-Human/Mouse phospho-STAT5 (Y694) PE. Cells in the lymphocyte gate were used for analysis. RIGHT: Intracellular staining of untreated (orange histogram) or 15-minute GM-CSF-treated (purple histogram) mouse thioglycolate-elicited peritoneal exudate cells with Anti-Human/... View More

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产品信息

12-9010-42

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

查看18篇已发表文章 18篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

SRBCZX

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2572671

产品详细信息

Description: This SRBCZX monoclonal antibody recognizes signal transducer and activator of transcription 5 (STAT5) when phosphorylated on tyrosine 694. STAT proteins are activated by ligand binding to receptors, such as cytokine receptors, that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. In response to cytokines that signal through the common gamma chain such as IL-2, IL-7, and IL-15, STAT5 is phosphorylated on tyrosine 694 by JAK1 and JAK3. Cytokines such as IL-3, IL-5, and GM-CSF that signal via the common beta chain induce STAT5 phosphorylation on tyrosine 694 by JAK 2. Phosphorylation of STAT5 on tyrosine 694 is essential for STAT5 dimer formation, nuclear translocation, and DNA binding activity.

Specificity of this SRBCZX clone was determined by ELISA and flow cytometry.

Applications Reported: This SRBCZX antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This SRBCZX antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol. Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used. All Protocols can be found in the Flow Cytometry Protocols: "Staining Intracellular Antigens for Flow Cytometry Protocol" located in the Best Protocols Section under the Resources tab online.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5 alpha is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of STAT5 alpha is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this protein in cells. STAT5 alpha, along with STAT5 beta, were identified in mouse. The amino acid sequences of STAT5 alpha and STAT5 beta show 96% sequence similarity, and both proteins are co expressed in most tissues in virgin and lactating mice. However, differential accumulation of STAT5 alpha and STAT5 beta mRNA has been reported for both muscle and mammary tissue. STAT5 alpha is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Heliyon

Duan M,Yu C,Yang Y,Fu Z,Liu C,Du J,Li M,Guo S,Yu X,Xu G,Mei Y,Wang L

Published figure using Phospho-STAT5 (Tyr694) monoclonal antibody (Product # 12-9010-42) in Flow Cytometry

Thu Jun 01 00:00:00 UTC 2023

Interleukin-23 receptor defines T helper 1-like regulatory T cells in oral squamous cell carcinoma.

Immunity, inflammation and disease

Li W,An N,Wang M,Liu X,Mei Z

12-9010-42 was used in Flow cytometry/Cell sorting to unveil Treg heterogeneity, thus deepening the understanding of Treg biology and tumor immunity in OSCC.

Thu Dec 01 00:00:00 UTC 2022

GM-CSF impairs erythropoiesis by disrupting erythroblastic island formation via macrophages.

Journal of translational medicine

Cao W,Fan W,Wang F,Zhang Y,Wu G,Shi X,Shi JX,Gao F,Yan M,Guo R,Li Y,Li W,Du C,Jiang Z

12-9010-42 was used in Flow Cytometry to document that GM-CSF impairs EBI formation in mice and humans.

Mon Jan 03 00:00:00 UTC 2022

GM-CSF impairs erythropoiesis by disrupting erythroblastic island formation via macrophages.

Journal of translational medicine

Cao W,Fan W,Wang F,Zhang Y,Wu G,Shi X,Shi JX,Gao F,Yan M,Guo R,Li Y,Li W,Du C,Jiang Z

12-9010-42 was used in Flow Cytometry to document that GM-CSF impairs EBI formation in mice and humans.

Mon Jan 03 00:00:00 UTC 2022

Ageing promotes early T follicular helper cell differentiation by modulating expression of RBPJ.

Aging cell

Webb LMC,Fra-Bido S,Innocentin S,Matheson LS,Attaf N,Bignon A,Novarino J,Fazilleau N,Linterman MA

12-9010-42 was used in Flow Cytometry to show increased formation of Tfh precursors (pre-Tfh) but no associated increase in germinal centre (GC)-Tfh cells in aged mice, suggesting age-driven promotion of only early Tfh cell differentiation.

Fri Jan 01 00:00:00 UTC 2021

Aged neutrophils form mitochondria-dependent vital NETs to promote breast cancer lung metastasis.

Journal for immunotherapy of cancer

Yang C,Wang Z,Li L,Zhang Z,Jin X,Wu P,Sun S,Pan J,Su K,Jia F,Zhang L,Wang H,Yu X,Shao X,Wang K,Qiu F,Yan J,Huang J

12-9010-42 was used in Flow Cytometry to investigate the existence and biological function of a rarely delved subset of neutrophils, named as tumor-associated aged neutrophils (Naged, CXCR4+CD62Llow), involved in premetastatic niche formation during breast cancer metastasis.

Fri Oct 01 00:00:00 UTC 2021

Aged neutrophils form mitochondria-dependent vital NETs to promote breast cancer lung metastasis.

Journal for immunotherapy of cancer

Yang C,Wang Z,Li L,Zhang Z,Jin X,Wu P,Sun S,Pan J,Su K,Jia F,Zhang L,Wang H,Yu X,Shao X,Wang K,Qiu F,Yan J,Huang J

Fri Oct 01 00:00:00 UTC 2021

Long-Term Programming of CD8 T Cell Immunity by Perinatal Exposure to Glucocorticoids.

Cell

Hong JY,Lim J,Carvalho F,Cho JY,Vaidyanathan B,Yu S,Annicelli C,Ip WKE,Medzhitov R

12-9010 was used in Flow cytometry/Cell sorting to demonstrate that perinatal stress can have long-term consequences on CD8 T cell immunity by altering HPA axis activity.

Thu Mar 05 00:00:00 UTC 2020

ILC3 deficiency and generalized ILC abnormalities in DOCK8-deficient patients.

Allergy

Eken A,Cansever M,Okus FZ,Erdem S,Nain E,Azizoglu ZB,Haliloglu Y,Karakukcu M,Ozcan A,Devecioglu O,Aksu G,Arikan Ayyildiz Z,Topal E,Karakoc Aydiner E,Kiykim A,Metin A,Cipe F,Kaya A,Artac H,Reisli I,Guner SN,Uygun V,Karasu G,Dönmez Altuntas H,Canatan H,Oukka M,Ozen A,Chatila TA,Keles S,Baris S,Unal E,Patiroglu T

12-9010 was used in Flow cytometry/Cell sorting to sought to test whether DOCK8 is required for the function and maintenance of ILC subsets in humans.

Wed Apr 01 00:00:00 UTC 2020

SRC-3 Functions as a Coactivator of T-bet by Regulating the Maturation and Antitumor Activity of Natural Killer Cells.

Cancer immunology research

Hu M,Lu Y,Qi Y,Zhang Z,Wang S,Xu Y,Chen F,Tang Y,Chen S,Chen M,Du C,Shen M,Wang F,Su Y,Deng Y,Wang J

12-9010-42 was used in Flow Cytometry to study the role of SRC-3 as a coactivator of T-bet in natural killer-cell biology and indicate that targeting SRC-3 may be a promising strategy to increase the tumor surveillance function of natural killer cells.

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Novel cytokine-antibody fusion protein, N-820, to enhance the functions of ex vivo expanded natural killer cells against Burkitt lymphoma.

Journal for immunotherapy of cancer

Chu Y,Nayyar G,Kham Su N,Rosenblum JM,Soon-Shiong P,Lee J,Safrit JT,Barth M,Lee D,Cairo MS

12-9010 was used in Flow cytometry/Cell sorting to provide the rationale for the development of a clinical trial of N-820 alone or in combination with endogenous or ex vivo expanded NK cells in patients with CD20+ B-NHL failing prior rituximab containing chemoimmunotherapy regimens.

Thu Oct 01 00:00:00 UTC 2020

Receptor-mediated dimerization of JAK2 FERM domains is required for JAK2 activation.

eLife

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Published figure using Phospho-STAT5 (Tyr694) monoclonal antibody (Product # 12-9010-42) in Flow Cytometry

Wed Jul 25 00:00:00 UTC 2018

CISH promoter polymorphism effects on T cell cytokine receptor signaling and type 1 diabetes susceptibility.

Molecular and cellular pediatrics

Seyfarth J,Ahlert H,Rosenbauer J,Baechle C,Roden M,Holl RW,Mayatepek E,Meissner T,Jacobsen M

12-9010 was used in Flow cytometry/Cell sorting to analyzed allele frequencies of three CISH SNPs (i.e., rs809451, rs414171, rs2239751) in a study of T1D patients (n = 260, onset age 5 years, duration > 10 years).

Tue Feb 06 00:00:00 UTC 2018

Aberrant plasma IL-7 and soluble IL-7 receptor levels indicate impaired T-cell response to IL-7 in human tuberculosis.

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Thu Jun 01 00:00:00 UTC 2017

Erythropoietin enhances Kupffer cell number and activity in the challenged liver.

Scientific reports

Gilboa D,Haim-Ohana Y,Deshet-Unger N,Ben-Califa N,Hiram-Bab S,Reuveni D,Zigmond E,Gassmann M,Gabet Y,Varol C,Neumann D

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Mon Sep 04 00:00:00 UTC 2017

Environmentally relevant concentrations of arsenite and monomethylarsonous acid inhibit IL-7/STAT5 cytokine signaling pathways in mouse CD3+CD4-CD8- double negative thymus cells.

Toxicology letters

Xu H,Lauer FT,Liu KJ,Hudson LG,Burchiel SW

12-9010 was used in Western Blotting to suggest that arsenic at environmentally-relevant doses suppresses early T-cell development through inhibition of IL-7 signaling.

Fri Apr 15 00:00:00 UTC 2016

Environmentally relevant concentrations of arsenite and monomethylarsonous acid inhibit IL-7/STAT5 cytokine signaling pathways in mouse CD3+CD4-CD8- double negative thymus cells.

Toxicology letters

Xu H,Lauer FT,Liu KJ,Hudson LG,Burchiel SW

12-9010 was used in Western Blotting to suggest that arsenic at environmentally-relevant doses suppresses early T-cell development through inhibition of IL-7 signaling.