Invitrogen

Phospho-STING (Ser366) Polyclonal Antibody

Phospho-STING (Ser366) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-STING (Ser366) Antibody (PA5-105674) in ICC/IF

Immunofluorescent analysis of Phospho-STING (Ser366) in HepG2 cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-STING (Ser366) polyclonal antibody (Product # PA5-105674) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 5... View More

Phospho-STING (Ser366) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-STING (Ser366) Antibody in Western Blot (WB)

Phospho-STING (Ser366) Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-105674

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human TMEM173(Accession Q86WV6), corresponding to amino acid residues around phosphorylated Ser366.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

sequential chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2817102

产品详细信息

Antibody detects endogenous levels of TMEM173/STING only when phosphorylated at Ser366.

靶标信息

STING is a 379 amino acid containing signaling protein that acts as an important component of innate immune signaling. This signaling protein is able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon (IFN-alpha and IFN-beta) and exert a potent anti-viral state following expression. STING may have translocon function, the translocon possibly being able to influence the induction of type I interferons and is also involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II) thus facilitating the detection of intracellular viral RNA species as well as B-form DNA and mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway. Generally seen in endoplasmic reticulum membrane, cell membrane and mitochondrion outer membrane, it is ubiquitously expressed in most of tissues.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Beclin-1 dependent autophagy improves renal outcomes following Unilateral Ureteral Obstruction (UUO) injury.

Frontiers in immunology

Lopez-Soler RI,Nikouee A,Kim M,Khan S,Sivaraman L,Ding X,Zang QS

PA5-105674 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to suggest that Unilateral Ureteral Obstruction (UUO) causes an insufficient, maladaptive renal autophagy, which triggered downstream activation of inflammatory STING pathway, production of cytokines, and pathological activation of ISR, eventually leading to the development of fibrosis.

Tue Mar 07 00:00:00 UTC 2023

Beclin-1 dependent autophagy improves renal outcomes following Unilateral Ureteral Obstruction (UUO) injury.

Frontiers in immunology

Lopez-Soler RI,Nikouee A,Kim M,Khan S,Sivaraman L,Ding X,Zang QS

Tue Mar 07 00:00:00 UTC 2023

PERK-STING-RIPK3 pathway facilitates cognitive impairment by inducing neuronal necroptosis in sepsis-associated encephalopathy.

CNS neuroscience & therapeutics

Xiaofeng G,You W,Qi J,Hongwei M,Zhongmin F,Shiquan W,Lixia D,Yuliang P,Zongping F,Xijing Z

PA5-105674 was used in Immunohistochemistry to investigate whether STING is involved in the underlying mechanism of Sepsis-associated encephalopathy (SAE).

Sat Apr 01 00:00:00 UTC 2023

PERK-STING-RIPK3 pathway facilitates cognitive impairment by inducing neuronal necroptosis in sepsis-associated encephalopathy.

CNS neuroscience & therapeutics

Xiaofeng G,You W,Qi J,Hongwei M,Zhongmin F,Shiquan W,Lixia D,Yuliang P,Zongping F,Xijing Z

PA5-105674 was used in Immunohistochemistry to investigate whether STING is involved in the underlying mechanism of Sepsis-associated encephalopathy (SAE).

Sat Apr 01 00:00:00 UTC 2023

Crosstalk between cytotoxic CD8+ T cells and stressed cardiomyocytes triggers development of interstitial cardiac fibrosis in hypertensive mouse hearts.

Frontiers in immunology

Brassington K,Kanellakis P,Cao A,Toh BH,Peter K,Bobik A,Kyaw T

PA5-105674 was used in Immunohistochemistry to show that crosstalk between cardiac cytotoxic memory CD8+ T cells and overly stressed cardiomyocytes is essential for development of non-ischemic hypertensive cardiac fibrosis.

Thu Dec 15 00:00:00 UTC 2022

Therapeutic modulation of phagocytosis in glioblastoma can activate both innate and adaptive antitumour immunity.

Nature communications

von Roemeling CA,Wang Y,Qie Y,Yuan H,Zhao H,Liu X,Yang Z,Yang M,Deng W,Bruno KA,Chan CK,Lee AS,Rosenfeld SS,Yun K,Johnson AJ,Mitchell DA,Jiang W,Kim BYS

PA5-105674 was used in Western Blot to show stimulation of glioma cell phagocytosis by blockage of anti-phagocytosis molecule CD47 is an ineffective process.

Fri Mar 20 00:00:00 UTC 2020

Therapeutic modulation of phagocytosis in glioblastoma can activate both innate and adaptive antitumour immunity.

Nature communications

von Roemeling CA,Wang Y,Qie Y,Yuan H,Zhao H,Liu X,Yang Z,Yang M,Deng W,Bruno KA,Chan CK,Lee AS,Rosenfeld SS,Yun K,Johnson AJ,Mitchell DA,Jiang W,Kim BYS

PA5-105674 was used in Western Blot to show stimulation of glioma cell phagocytosis by blockage of anti-phagocytosis molecule CD47 is an ineffective process.

Fri Mar 20 00:00:00 UTC 2020

生物信息学

蛋白别名: endoplasmic reticulum IFN stimulator; Endoplasmic reticulum interferon stimulator; ERIS; hMITA; hSTING; Mediator of IRF3 activation; mitochondrial mediator of IRF3 activation; mitochondrial transmembrane protein 173; MMITA; mSTING; N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner; rSTING; Stimulator of interferon genes protein; tm173; Transmembrane protein 173

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基因别名: 2610307O08Rik; ERIS; hMITA; hSTING; MITA; MPYS; NET23; RGD1562552; SAVI; STING; STING1; TMEM173

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UniProt ID: (Human) Q86WV6, (Mouse) Q3TBT3, (Rat) F1M391

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Entrez Gene ID: (Human) 340061, (Mouse) 72512, (Rat) 498840

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功能

protein binding transcription factor binding protein kinase binding ubiquitin protein ligase binding cyclic-di-GMP binding identical protein binding protein homodimerization activity cyclic-GMP-AMP binding nucleotide binding

参与通路