Invitrogen

Phospho-VEGF Receptor 2 (Tyr1054, Tyr1059) Polyclonal Antibody

Phospho-VEGF Receptor 2 (Tyr1054, Tyr1059) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-VEGF Receptor 2 (Tyr1054, Tyr1059) Antibody (44-1047G) in ICC/IF

Immunofluorescence analysis of Phospho-VEGF Receptor 2 pTyr1054/pTyr1059 was done on 90% confluent log phase HUVEC cell treated with 100 ng of VEGF for 30 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-VEGF Receptor 2 pTyr1054/pTyr1059 Rabb... View More

Phospho-VEGF Receptor 2 (Tyr1054, Tyr1059) Antibody in Western Blot (WB)

Phospho-VEGF Receptor 2 (Tyr1054, Tyr1059) Antibody

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产品信息

44-1047G

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫细胞化学 (ICC/IF)

1:250

流式细胞分析 (Flow)

1:20

其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human VEGFR2 that contains tyrosines 1054 and 1059. The sequence is conserved among multiple species including mouse and rat.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533557

产品详细信息

On western blotting this antibody detects a protein with the apparent MW of 150 kDa. Positive controls used Porcine aortic endothelial (PAE) cells transfected with a chimeric receptor consisting of the extracellular domain of the CSF-1 receptor coupled to the transmembrane and cytoplasmic domains of the mouse VEGFR2, or 293T cells transfected with full length VEGFR2 and stimulated with 10 ng/mL VEGF for 5 minutes.

VEGFR3 has not been tested, but is expected to react with this antibody.

靶标信息

KDR (FLK1, VEGFR2) is a type III receptor tyrosine kinase. It binds vascular endothelial growth factor with high affinity in vitro, and is expressed early in development by endothelial cell precursors. FLK1 (VEGF Receptor 2) encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc. Mutations of FLK1 (VEGF Receptor 2) are implicated in infantile capillary hemangiomas.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Critical role of mitogen-inducible gene 6 in restraining endothelial cell permeability to maintain vascular homeostasis.

Journal of cell communication and signaling

Xing L,Huang G,Chen R,Huang L,Liu J,Ren X,Wang S,Kuang H,Kumar A,Kim JK,Jiang Q,Li X,Lee C

44-1047G was used in Western Blotting to show for the first time a critical role of MIG6 in limiting vascular permeability.

Wed Mar 01 00:00:00 UTC 2023

VLA-4 phosphorylation during tumor and immune cell migration relies on its coupling to VEGFR2 and CXCR4 by syndecan-1.

Journal of cell science

Jung O,Beauvais DM,Adams KM,Rapraeger AC

44-1047G was used in Western Blotting to demonstrate that VEGFR2 acts as a scaffold for the entire machinery necessary for phosphorylation of VLA-4 on S988.

Mon Oct 28 00:00:00 UTC 2019

Epidermal-specific deletion of TC-PTP promotes UVB-induced epidermal cell survival through the regulation of Flk-1/JNK signaling.

Cell death & disease

Baek M,Kim M,Lim JS,Morales LD,Hernandez J,Mummidi S,Williams-Blangero S,Jang IS,Tsin AT,Kim DJ

44-1047G was used in Western Blotting to suggest that T-cell protein tyrosine phosphatase is protective against UVB-induced keratinocyte cell damage by promoting apoptosis via negative regulation of Flk-1/JNK survival signalling.

Thu Jun 28 00:00:00 UTC 2018

Heparanase-induced shedding of syndecan-1/CD138 in myeloma and endothelial cells activates VEGFR2 and an invasive phenotype: prevention by novel synstatins.

Oncogenesis

Jung O,Trapp-Stamborski V,Purushothaman A,Jin H,Wang H,Sanderson RD,Rapraeger AC

44-1047G was used in Western Blotting to study the mechanism through which heparanase modulates syndecan-1 function to promote both tumour cell invasion and angiogenesis.

Mon Feb 29 00:00:00 UTC 2016

Intramembrane binding of VE-cadherin to VEGFR2 and VEGFR3 assembles the endothelial mechanosensory complex.

The Journal of cell biology

Coon BG,Baeyens N,Han J,Budatha M,Ross TD,Fang JS,Yun S,Thomas JL,Schwartz MA

44-1047G was used in western blot to study the role of VE-cadherin in fluid shear stress

Mon Mar 30 00:00:00 UTC 2015

The docking protein FRS2α is a critical regulator of VEGF receptors signaling.

Proceedings of the National Academy of Sciences of the United States of America

Chen PY,Qin L,Zhuang ZW,Tellides G,Lax I,Schlessinger J,Simons M

44-1047G was used in western blot to show that FRS2α is a component of the VEGF receptors.

Tue Apr 15 00:00:00 UTC 2014

The docking protein FRS2α is a critical regulator of VEGF receptors signaling.

Proceedings of the National Academy of Sciences of the United States of America

Chen PY,Qin L,Zhuang ZW,Tellides G,Lax I,Schlessinger J,Simons M

44-1047G was used in western blot to show that FRS2α is a component of the VEGF receptors.

Tue Apr 15 00:00:00 UTC 2014

Investigation of the potential utility of a linomide analogue for treatment of choroidal neovascularization.

Experimental eye research

Abdel-Rahman MH,Yang Y,Salem MM,Meadows S,Massengill JB,Li PK,Davidorf FH

Wed Dec 01 00:00:00 UTC 2010

生物信息学

蛋白别名: CD309; CD309 antigen; Fetal liver kinase 1; fetal liver kinase-1; FLK-1; KDR; Kinase insert domain receptor; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase NYK; Protein-tyrosine kinase receptor flk-1; sCD309; soluble CD309; soluble vascular endothelial growth factor receptor 2; soluble VEGFR 2; soluble VEGFR2; tyrosine kinase growth factor receptor; Vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor- 2; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; VEGF R2; VEGF receptor-2; VEGFR-2

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基因别名: 6130401C07; CD309; Flk-1; FLK1; KDR; Krd-1; Ly73; sVEGFR-2; VEGFR; VEGFR-2; VEGFR2

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UniProt ID: (Human) P35968, (Mouse) P35918

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Entrez Gene ID: (Human) 3791, (Mouse) 16542

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