Invitrogen

Phospho-Vinculin (Tyr1065) Polyclonal Antibody

Phospho-Vinculin (Tyr1065) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-Vinculin (Tyr1065) Antibody (44-1078G) in ICC/IF

Immunofluorescent analysis of Phospho-Vinculin pTyr1065 Antibody was done on 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Vinculin pTyr1065 Antibody (Product # 44-1078G) at 1:250 dilution in 1% BSA and incubat... View More

Phospho-Vinculin (Tyr1065) Antibody in Western Blot (WB)

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产品信息

44-1078G

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看6篇已发表文章 6篇已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:250

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Chicken, Human

已发表种属

Dog, Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human vinculin that contains tyrosine 1065. The sequence is conserved in mouse and chicken.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Antigen affinity chromatography

保存液

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533568

靶标信息

Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The proprotein convertase furin inhibits IL-13-induced inflammation in airway smooth muscle by regulating integrin-associated signaling complexes.

American journal of physiology. Lung cellular and molecular physiology

Wu Y,Huang Y,Zhang W,Gunst SJ

44-1078G was used in Western Blotting to demonstrate that furin inhibits inflammation in airway smooth muscle induced by IL-13 and that the anti-inflammatory effects of furin are mediated by activating integrin proteins and integrin-associated signaling complexes that regulate Akt-mediated pathways to the nucleus.

Thu Jul 01 00:00:00 UTC 2021

FAK Shutdown: Consequences on Epithelial Morphogenesis and Biomarker Expression Involving an Innovative Biomaterial for Tissue Regeneration.

International journal of molecular sciences

Wang X,Steinberg T,Dieterle MP,Ramminger I,Husari A,Tomakidi P

44-1078G was used in Immunohistochemistry (Frozen) to indicate the mechanistic role of FAK in epithelial morphogenesis by simultaneously considering prospective biomaterial-based epithelial regenerative approaches.

Fri Sep 10 00:00:00 UTC 2021

S100A4 is activated by RhoA and catalyses the polymerization of non-muscle myosin, adhesion complex assembly and contraction in airway smooth muscle.

The Journal of physiology

Zhang W,Gunst SJ

44-1078G was used in Western Blot to test the hypothesis that S100A4 contributes to the regulation of contraction in airway smooth muscle by regulating a pool of non-muscle myosin II at the cell cortex.

Thu Oct 01 00:00:00 UTC 2020

Inhibition of Smooth Muscle Cell Proliferation and Migration by a Talin Modulator Attenuates Neointimal Formation after Femoral Arterial Injury.

Korean circulation journal

Lim IR,Kim C,Jung JW,Kim JH,Hong SJ

44-1078G was used in Western Blotting to demonstrate the inhibitory effects of a talin modulator on SMC proliferation and migration, and that were associated with downregulation of signaling pathways, resulting in the attenuation of neointimal formation in ApoE KO mice.

Wed Jul 01 00:00:00 UTC 2020

Inhibition of Smooth Muscle Cell Proliferation and Migration by a Talin Modulator Attenuates Neointimal Formation after Femoral Arterial Injury.

Korean circulation journal

Lim IR,Kim C,Jung JW,Kim JH,Hong SJ

Wed Jul 01 00:00:00 UTC 2020

The Role of Hypoxia in Corneal Extracellular Matrix Deposition and Cell Motility.

Anatomical record (Hoboken, N.J. : 2007)

Onochie OE,Onyejose AJ,Rich CB,Trinkaus-Randall V

44-1078G was used in Immunocytochemistry-immunoflourescence to demonstrate that low-oxygenated environments modify the composition of the extracellular matrix, basal lamina stiffness, and focal adhesion dynamics, leading to alterations in the function of the cornea.