Invitrogen

Phospho-WEE1 (Ser139) Polyclonal Antibody

Phospho-WEE1 (Ser139) Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 4

Phospho-WEE1 (Ser139) Antibody (PA5-105559) in ICC/IF

Immunocytochemistry analysis of Phospho-WEE1 (Ser139) in Hela cells (4h of LPS treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-WEE1 (Ser139) polyclonal antibody (Product # PA5-105559) 1:200 and mouse anti-beta tubulin antibody (1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat ... View More

Phospho-WEE1 (Ser139) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-WEE1 (Ser139) Antibody in Western Blot (WB)

Product Image Gallery

产品信息

PA5-105559

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human WEE1(Accession P30291), corresponding to amino acid residues around phosphorylated Ser139.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

sequential chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2816987

产品详细信息

Antibody detects endogenous levels of WEE1 only when phosphorylated at Ser139.

靶标信息

WEE1, which is classified as a Ser/Thr kinase by sequence analysis, has been shown to inactivate multiple CDKs by tyrosine phosphorylation. Wee1 protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

仅用于科研。不用于诊断过程。未经明确授权不得转售。