Invitrogen

Phospho-gamma-H2AX (Ser139) Recombinant Rabbit Monoclonal Antibody (RM224)

Phospho-gamma-H2AX (Ser139) Antibody in Immunocytochemistry (ICC/IF)

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Phospho-gamma-H2AX (Ser139) Antibody (MA5-33062) in ICC/IF

Immunocytochemistry analysis of HeLa cells, using Phospho-gamma-H2AX (Ser139) Recombinant Monoclonal antibody (Product # MA5-33062) (red). Actin filaments have been labeled with fluorescein phalloidin (green).

Phospho-gamma-H2AX (Ser139) Antibody in Western Blot (WB)

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产品信息

MA5-33062

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-2 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

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免疫细胞化学 (ICC/IF)

0.5-2 µg/mL

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酶联免疫吸附实验 (ELISA)

0.2-1 µg/mL

Luminex (LUM)

0.1-1 µg/mL

产品规格

种属反应

All, Human, Mouse, Rat

已发表种属

Human, Mouse, Rhesus monkey

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

RM224

抗原

A phospho-peptide corresponding to Phospho-Histone H2AX (Ser139).

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1% BSA, 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2810155

产品详细信息

Specificity: reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

H2AX is a variant of the H2A protein family and is a component of the histone octomer in nucleosomes. H2AX is phosphorylated by kinases such as ataxia telangiectasia mutated (ATM) and ATM-Rad3-related (ATR) in the PI3K pathway. Gamma-H2AX is the first step in recruiting and localizing DNA repair proteins. DSBs can be induced by mechanisms such as ionizing radiation or cytotoxic agents and subsequently, gamma-H2AX foci quickly form. These foci represent the DSBs in a 1:1 manner and can be used as a biomarker for damage. The visualization of gamma-H2AX by can allow the assessment of DNA damage, related DNA damage proteins and DNA repair. Gamma-H2AX has applications in the detection of genomic damage caused by cytotoxic chemical agents and environmental and physical damage.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Sting and p53 DNA repair pathways are compromised in Alzheimer's disease.

Scientific reports

Nelson TJ,Xu Y

MA5-33062 was used in Western Blot to suggest that oxidation of p53 by ROS could inhibit the DDR and decrease its ability to orchestrate DSB repair by altering the oligomerization state of p53.

Tue May 23 00:00:00 UTC 2023

Evidence That SARS-CoV-2 Induces Lung Cell Senescence: Potential Impact on COVID-19 Lung Disease.

American journal of respiratory cell and molecular biology

Lipskaia L,Maisonnasse P,Fouillade C,Sencio V,Pascal Q,Flaman JM,Born E,Londono-Vallejo A,Le Grand R,Bernard D,Trottein F,Adnot S

MA5-33062 was used immunohistochemistry (paraffin) provide evidence that SARS-CoV-2 induces lung-cell senescence.

Sat Jan 01 00:00:00 UTC 2022

A multifunctional AIE gold cluster-based theranostic system: tumor-targeted imaging and Fenton reaction-assisted enhanced radiotherapy.

Journal of nanobiotechnology

Hua Y,Wang Y,Kang X,Xu F,Han Z,Zhang C,Wang ZY,Liu JQ,Zhao X,Chen X,Zang SQ

MA5-33062 was used in Immunocytochemistry-immunofluorescence to report the fabrication of cyclic RGD peptide (cRGD) modified Au4-iron oxide nanoparticle (Au4-IO NP-cRGD) based on aggregation-induced emission (AIE) as a multifunctional theranostic system.

Mon Dec 20 00:00:00 UTC 2021

p53 drives a transcriptional program that elicits a non-cell-autonomous response and alters cell state in vivo.

Proceedings of the National Academy of Sciences of the United States of America

Moyer SM,Wasylishen AR,Qi Y,Fowlkes N,Su X,Lozano G

MA5-33062 was used in Immunohistochemistry to investigate the molecular, morphological, and physiological consequences of activation of p53 cascade.

Tue Sep 22 00:00:00 UTC 2020

生物信息学

蛋白别名: gamma H2A X; gamma H2A.X; gamma-H2A.X; gh2ax; H2A histone family, member X; H2a/x; H2AX histone; histone 5 protein 2ax; Histone H2A.X; Histone H2AX

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基因别名: AW228881; gammaH2ax; H2A.X; H2A/X; H2AFX; H2AX; Hist5-2ax; RGD1566119

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UniProt ID: (Human) P16104, (Mouse) P27661

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Entrez Gene ID: (Human) 3014, (Rat) 500987, (Mouse) 15270

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功能

DNA binding damaged DNA binding protein binding enzyme binding histone binding protein heterodimerization activity molecular_function histone

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