Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
图: 1 / 13
Phospho-nNOS (Ser1417) Antibody (PA1-032) in ICC/IF
Immunofluorescent analysis of Phospho-nNOS pSer1417 (green) showing staining in the cytoplasm and nucleus of SH-SY5Y cells treated with 100 ng/mL nocodazole (left) and untreated SH-SY5Y cells (right). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospho-nNOS pSer1417 polyclonal ... View More
产品信息
PA1-032
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Rat
已发表种属
Human,
Mouse,
Rabbit,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic phosphopeptide corresponding to residues C N(1411) R L R S E (pS) I A F I E E S K(1425) of human nNOS.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_325020
产品详细信息
PA1-032 detects phosphorylated nNOS (Ser 1417) from rat and human samples.
PA1-032 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~140 kDa protein representing recombinant rat phospho-nNOS (Ser 1417).
The PA1-032 immunogen is a synthetic phosphopeptide to residues C N(1411) R L R S E (pS) I A F I E E S K(1425) of human nNOS. The peptide sequence is 100% conserved in rat, frog, and mouse protein. This peptide (Cat. # PEP-188) is available for use in neutralization and control experiments.
靶标信息
NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: BNOS; bNOS1; Brain NOS; Constitutive NOS; N-NOS; NC-NOS; Neuronal NOS; nitric oxidase synthase; nitric oxide synthase 1 (neuronal); Nitric oxide synthase, brain; nNOS; NOS Type 1; NOS type I; Peptidyl-cysteine S-nitrosylase NOS1; Phospho-bNOS; Phospho-Brain NOS
基因别名: Bnos; IHPS1; N-NOS; NC-NOS; nNOS; NOS; NOS1
UniProt ID: (Human) P29475, (Rat) P29476
Entrez Gene ID: (Human) 4842, (Rat) 24598
nitric-oxide synthase activity
iron ion binding
protein binding
calmodulin binding
FMN binding
sodium channel regulator activity
enzyme binding
heme binding
tetrahydrobiopterin binding
arginine binding
protein homodimerization activity
ion channel binding
cadmium ion binding
flavin adenine dinucleotide binding
NADP binding
scaffold protein binding
NADPH-hemoprotein reductase activity
amino acid binding
ATPase binding
response to hypoxia
regulation of sodium ion transport
arginine catabolic process
nitric oxide biosynthetic process
muscle contraction
striated muscle contraction
nitric oxide mediated signal transduction
female pregnancy
aging
regulation of heart contraction
negative regulation of cell proliferation
response to heat
response to organonitrogen compound
response to lead ion
response to organic cyclic compound
response to activity
peptidyl-cysteine S-nitrosylation
positive regulation of guanylate cyclase activity
response to nutrient levels
response to lipopolysaccharide
negative regulation of peptidyl-serine phosphorylation
response to vitamin E
multicellular organismal response to stress
positive regulation of histone acetylation
response to nicotine
exogenous drug catabolic process
negative regulation of apoptotic process
negative regulation of potassium ion transport
response to peptide hormone
response to estrogen
response to ethanol
negative regulation of blood pressure
negative regulation of heart contraction
positive regulation of transcription, DNA-templated
negative regulation of vasoconstriction
positive regulation of vasodilation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of insulin secretion
behavioral response to cocaine
negative regulation of hydrolase activity
negative regulation of cytosolic calcium ion concentration
negative regulation of serotonin uptake
negative regulation of calcium ion transport
regulation of sensory perception of pain
oxidation-reduction process
cellular response to mechanical stimulus
cellular response to growth factor stimulus
response to nitric oxide
cellular response to epinephrine stimulus
positive regulation of the force of heart contraction
positive regulation of long-term synaptic potentiation
positive regulation of adrenergic receptor signaling pathway involved in heart process
positive regulation of neuron death
positive regulation of sodium ion transmembrane transport
myoblast fusion
negative regulation of calcium ion transport into cytosol
neurotransmitter biosynthetic process
cell redox homeostasis
regulation of neurogenesis
regulation of cardiac muscle contraction
regulation of ryanodine-sensitive calcium-release channel activity
negative regulation of adrenergic receptor signaling pathway involved in heart process
regulation of calcium ion transmembrane transport via high voltage-gated calcium channel
regulation of cardiac conduction
