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Plexin-B2 Antibody (14-5665-82) in Flow
Staining of the PDV cell line with 0.25 µg of Armenian Hamster IgG Isotype Control Purified (Product # 14-4888-81) (open histogram) or 0.25 µg of Anti-Mouse Plexin-B2 Purified (filled histogram) followed by Anti-Armenian Hamster IgG Biotin (Product # 13-4113-85) and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
产品信息
14-5665-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Mouse
宿主/亚型
Armenian hamster
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
eBio3E7 (3E7)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_763626
产品详细信息
Description: Plexin-B2 is a member of the Semaphorin receptor family. The semaphorin family consists of membrane and secreted proteins involved in attraction and repulsion of neuronal guidance cues. Plexin-B1 is a transmembrane protein that can be cleaved coverting the single chain presursor into a heterodimeric receptor. It has been shown to bind Sema4D/CD100 and is involved in axon development in mouse embryos. Sema4D also plays a role in the immune system interacting with CD72. Plexin-B2's possible role in the immune system has not been characterized.
Applications Reported: This eBio3E7 (3E7) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining.
Applications Tested: This eBio3E7 (3E7) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Plexin-B2 is a member of the Semaphorin receptor family. The semaphorin family consists of membrane and secreted proteins involved in attraction and repulsion of neuronal guidance cues. Plexin-B1 is a transmembrane protein that can be cleaved converting the single chain precursor into a heterodimeric receptor. It has been shown to bind Sema4D/CD100 and is involved in axon development in mouse embryos. Sema4D also plays a role in the immune system interacting with CD72. Plexin-B2's possible role in the immune system has not been characterized.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: differentially expressed in brain tumors; MM1KIAA0315dJ402G11.3; Plexin-B2
基因别名: Debt; plexin-B2; Plxnb2
UniProt ID: (Mouse) B2RXS4
Entrez Gene ID: (Mouse) 140570
neural tube closure
regulation of protein phosphorylation
signal transduction
multicellular organism development
neuroblast proliferation
brain development
regulation of cell shape
regulation of GTPase activity
positive regulation of axonogenesis
semaphorin-plexin signaling pathway
regulation of neuron migration
