Invitrogen

Presenilin 1 Polyclonal Antibody

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抗体检测数据 (5)

Presenilin 1 Antibody in Immunocytochemistry (ICC/IF)

Presenilin 1 Antibody in Immunocytochemistry (ICC/IF)

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Presenilin 1 Antibody (PA5-119872) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of of Presenilin 1/PS-1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with Presenilin 1 Polyclonal Antibody (Product # PA5-119872) at a dilution of 1:50 for 1 hour at room temperature,... View More

Presenilin 1 Antibody in Immunocytochemistry (ICC/IF)

Presenilin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Presenilin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Presenilin 1 Antibody in Western Blot (WB)

Presenilin 1 Antibody in Western Blot (WB)

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Presenilin 1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of of Presenilin 1/PS-1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with Presenilin 1 Polyclonal Antibody (Product # PA5-119872) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

Presenilin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of paraffin-embedded human small intestine tissue using Presenilin 1 Polyclonal Antibody (Product # PA5-119872). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the Presenilin 1 antibody at a dilution of 1:400 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Presenilin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of paraffin-embedded mouse testis tissue using Presenilin 1 Polyclonal Antibody (Product # PA5-119872). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the Presenilin 1 antibody at a dilution of 1:400 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Presenilin 1 Antibody in Western Blot (WB) — Western blot analysis of Presenilin 1/PS-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Presenilin 1 Polyclonal Antibody (Product # PA5-119872) at 1:1,000 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate. Lane 2: Raji cell lysate. Lane 3: MCF-7 cell lysate. Lane 4: Daudi cell lysate.

Presenilin 1 Antibody in Western Blot (WB) — Western blot analysis of Presenilin 1/PS-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Presenilin 1 Polyclonal Antibody (Product # PA5-119872) at 1:2,000 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse liver tissue lysate. Lane 2: Rat liver tissue lysate.

产品信息

PA5-119872

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:500

-

免疫细胞化学 (ICC/IF)

1:50-1:100

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide within human Presenilin 1 aa 330-380

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2913444

产品详细信息

Positive Control: NIH/3T3 cell lysate, Raji cell lysate, MCF-7 cell lysate, Daudi cell lysate, mouse liver tissue lysate, rat liver tissue lysate, MCF-7, human small intestine tissue, mouse testis tissue.

Subcellular Location: Endoplasmic reticulum, Endoplasmic reticulum membrane, Golgi apparatus membrane, Cell membrane, Early endosome, Early endosome membrane, Cytoplasmic granule, growth cone, neuron projection, axon, synapse.

靶标信息

Presenilin1 was initially identified a marker of susceptibility to early-onset Alzheimer's disease. In addition to PEN2, nicastrin and APH-1, Presenilin1 forms the -gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer's disease. Like the tumor necrosis factor-alpha-converting enzyme (TACE) and the beta-site cleavage enzyme (BACE) protease families, -gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and -gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and -gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer's disease. Multiple isoforms of presenilin1 are known to exist.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Alzheimer Disease 3; Minilin; Presenilin-1; presenilin1; Protein S182; PS-1; S182 protein; Senilin 1

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基因别名: AD3; Ad3h; FAD; PS-1; PS1; PSEN1; PSNL1; S182

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UniProt ID: (Human) P49768, (Rat) P97887

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Entrez Gene ID: (Human) 5663, (Rat) 29192, (Mouse) 19164

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endopeptidase activity aspartic-type endopeptidase activity calcium channel activity protein binding beta-catenin binding PDZ domain binding cadherin binding peptidase activity hydrolase activity aspartic endopeptidase activity, intramembrane cleaving aspartic protease

autophagosome assembly negative regulation of transcription from RNA polymerase II promoter activation of MAPKK activity blood vessel development cell fate specification somitogenesis neuron migration positive regulation of receptor recycling heart looping hematopoietic progenitor cell differentiation T cell activation involved in immune response neural retina development protein glycosylation membrane protein ectodomain proteolysis mitochondrial transport cellular response to DNA damage stimulus response to oxidative stress negative regulation of epidermal growth factor-activated receptor activity Notch signaling pathway Notch receptor processing memory post-embryonic development protein transport L-glutamate transport choline transport synaptic vesicle targeting single organismal cell-cell adhesion protein processing cerebral cortex cell migration Cajal-Retzius cell differentiation dorsal/ventral neural tube patterning embryonic limb morphogenesis positive regulation of proteasomal ubiquitin-dependent protein catabolic process endoplasmic reticulum calcium ion homeostasis beta-amyloid formation intracellular signal transduction regulation of phosphorylation amyloid precursor protein catabolic process myeloid dendritic cell differentiation positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of catalytic activity regulation of protein binding positive regulation of MAP kinase activity negative regulation of neuron apoptotic process skin morphogenesis positive regulation of transcription, DNA-templated regulation of synaptic plasticity thymus development neuron development skeletal system morphogenesis brain morphogenesis epithelial cell proliferation negative regulation of axonogenesis positive regulation of coagulation T cell receptor signaling pathway neuron apoptotic process negative regulation of ubiquitin-protein transferase activity smooth endoplasmic reticulum calcium ion homeostasis regulation of synaptic transmission, glutamatergic canonical Wnt signaling pathway regulation of resting membrane potential positive regulation of dendritic spine development calcium ion transmembrane transport negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic signaling pathway negative regulation of protein phosphorylation positive regulation of protein phosphorylation myeloid leukocyte differentiation negative regulation of protein kinase activity proteolysis calcium ion transport cellular calcium ion homeostasis autophagy regulation of epidermal growth factor-activated receptor activity brain development heart development learning or memory cerebral cortex development neurogenesis neuron differentiation forebrain development segmentation locomotion eye photoreceptor cell development cellular protein metabolic process positive regulation of protein kinase activity beta-amyloid metabolic process protein maturation apoptotic process cell adhesion embryo development anagen