Invitrogen

RAB7 Polyclonal Antibody

RAB7 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 13

RAB7 Antibody (PA5-52369) in ICC/IF

Immunofluorescent staining of RAB7 in human cell line U-251 MG using a RAB7 Polyclonal Antibody (Product # PA5-52369) shows localization to lysosomes.

RAB7 Antibody in Immunohistochemistry (IHC)

RAB7 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Product Image Gallery

RAB7 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A-431 cells were transfected with, control siRNA, RAB7 siRNA probe #1, and RAB7 siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using Anti-RAB7 Polyclonal Antibody (Product # PA5-52369) used at a dilution of 0.4 µg/mL. Anti-GAPDH was used as a loading control. {KD}

产品信息

PA5-52369

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human RAB7. Recombinant protein control fragment (Product #RP-89503).

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.20 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2646252

产品详细信息

Immunogen sequence: CVLVFDVTAP NTFKTLDSWR DEFLIQASPR DPENFPFVVL GNKIDLENRQ VATKRAQAWC YSKNNIPYFE TSAKEAINVE QAFQTIARNA LKQETEVELY NEFPEPIKLD KNDRAK

Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 99%.

靶标信息

RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Dysregulation of endocytic machinery and ACE2 in small airways of smokers and COPD patients can augment their susceptibility to SARS-CoV-2 (COVID-19) infections.

American journal of physiology. Lung cellular and molecular physiology

Eapen MS,Lu W,Hackett TL,Singhera GK,Thompson IE,McAlinden KD,Hardikar A,Weber HC,Haug G,Wark PAB,Chia C,Sohal SS

PA5-52369 was used in Immunohistochemistry to identify the presence of SARS-CoV-2t host attachment receptor ACE2 within smokers andCOPD lungs.

Fri Jan 01 00:00:00 UTC 2021

Dysregulation of endocytic machinery and ACE2 in small airways of smokers and COPD patients can augment their susceptibility to SARS-CoV-2 (COVID-19) infections.

American journal of physiology. Lung cellular and molecular physiology

Eapen MS,Lu W,Hackett TL,Singhera GK,Thompson IE,McAlinden KD,Hardikar A,Weber HC,Haug G,Wark PAB,Chia C,Sohal SS

PA5-52369 was used in Immunohistochemistry to identify the presence of SARS-CoV-2t host attachment receptor ACE2 within smokers andCOPD lungs.

Fri Jan 01 00:00:00 UTC 2021

α- Linolenic acid modulates phagocytosis and endosomal pathways of extracellular Tau in microglia.

Cell adhesion & migration

Desale SE,Chinnathambi S

PA5-52369 was used in Immunocytochemistry-immunoflourescence to suggest that microglia could be influenced to reduce extracellular Tau seed with dietary fatty acids.

Wed Dec 01 00:00:00 UTC 2021

α- Linolenic acid modulates phagocytosis and endosomal pathways of extracellular Tau in microglia.

Cell adhesion & migration

Desale SE,Chinnathambi S

PA5-52369 was used in Immunocytochemistry-immunoflourescence to suggest that microglia could be influenced to reduce extracellular Tau seed with dietary fatty acids.

Wed Dec 01 00:00:00 UTC 2021

α- Linolenic acid modulates phagocytosis and endosomal pathways of extracellular Tau in microglia.

Cell adhesion & migration

Desale SE,Chinnathambi S

PA5-52369 was used in Immunocytochemistry-immunoflourescence to suggest that microglia could be influenced to reduce extracellular Tau seed with dietary fatty acids.

Wed Dec 01 00:00:00 UTC 2021

Major Determinants of Airway Epithelial Cell Sensitivity to S. aureus Alpha-Toxin: Disposal of Toxin Heptamers by Extracellular Vesicle Formation and Lysosomal Degradation.

Toxins

Möller N,Ziesemer S,Hentschker C,Völker U,Hildebrandt JP

Published figure using RAB7 polyclonal antibody (Product # PA5-52369) in Western Blot

Wed Feb 24 00:00:00 UTC 2021

Nanoparticle-based "Two-pronged" approach to regress atherosclerosis by simultaneous modulation of cholesterol influx and efflux.

Biomaterials

He H,Wang J,Yannie PJ,Korzun WJ,Yang H,Ghosh S

PA5-52369 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to investigate cholesterol efflux and influx in atherosclerosis regression.

Sun Nov 01 00:00:00 UTC 2020

生物信息学

蛋白别名: rab-7; RAB7, member RAS oncogene family; Ras-associated protein RAB7; Ras-related protein BRL-RAS; Ras-related protein p23; Ras-related protein Rab-7a

查看更多收起

基因别名: PRO2706; RAB7; RAB7A

查看更多收起

UniProt ID: (Human) P51149, (Mouse) P51150, (Rat) P09527

查看更多收起

Entrez Gene ID: (Human) 7879, (Mouse) 19349, (Rat) 29448

查看更多收起

功能

GTPase activity protein binding GTP binding GDP binding Rac GTPase binding nucleotide binding

参与通路