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RAD52 Antibody (MA5-31888) in ICC/IF
Immunocytochemistry analysis of RAD52 in HeLa cells (green). Sample was incubated with RAD52 monoclonal antibody (Product # MA5-31888) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .
产品信息
MA5-31888
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
5E11E7
抗原
Purified recombinant fragment of human RAD52 (AA: 269-418) expressed in E. coli.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2787511
产品详细信息
MA5-31888 has been tested in indirect ELISA.
靶标信息
DNA double-strand breaks can be generated by exposure to ionizing radiation or through endogenous cellular reactions. Double-strand breaks are generally thought to be repaired via one of two distinct mechanisms: homologous recombination (HR) or non-homologous end joining (NHEJ). Homologous recombination requires the activity of the members of the RAD52 epistasis group, which includeRAD50, RAD51, RAD52, RAD54, RDH54/TID1, RAD55, RAD57, RAD59, MRE11, and XRS2. RAD52 can catalyze strand annealing reactions, and can form oligomers that attach to ends of single-stranded DNA.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DNA repair protein RAD52 homolog; recombination protein RAD52; rhabdomyosarcoma antigen MU-RMS-40.23
基因别名: RAD52
UniProt ID: (Human) P43351
Entrez Gene ID: (Human) 5893
