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RAE1 delta Antibody (14-5756-81) in Flow
Staining of dendritic cell line with 0.125 µg of Mouse IgG1 kappa Isotype Control Purified (cat. 14-4714) (open histogram) or 0.125 µg of Anti-Mouse RAE1 delta Purified (filled histogram) followed by F(ab')2 Anti-Mouse IgG PE (cat. 12-4012). Total viable cells were used for analysis.
产品信息
14-5756-81
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Human,
Mouse
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
RD-41
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_1210509
产品详细信息
Description: The RD-41 monoclonal antibody reacts with the murine Rae1delta. Rae-1delta is one of several known murine NKG2D ligands that include RAE-1 molecules (alpha, beta, ε, gamma and delta), H60 and MULT-1. Expression of NKG2D ligands is low or absent on normal adult tissues. However, stressed or transformed cells express NKG2D ligands which in turn activates NK cells tumoricidal activity through NKG2D. Until now, the expression of NKG2D ligands has been mainly studied with NKG2D tetramers which recognizes all NKG2D ligands. The RD-41 antibody has been reported to block tetramer staining.
Applications Reported: This RD-41 antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections and ELISA.
Applications Tested: This RD-41 antibody has been tested by flow cytometric analysis of dendritic cell line. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Rae-1 delta is one of several known murine NKG2D ligands that include RAE-1 molecules, H60 and MULT-1. Expression of NKG2D ligands is low or absent on normal adult tissues. However, stressed or transformed cells express NKG2D ligands which in turn activates NK cells tumoricidal activity through NKG2D. Until now, the expression of NKG2D ligands has been mainly studied with NKG2D tetramers which recognizes all NKG2D ligands.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: RAE-1-delta; retinoic acid early transcript 1D; Retinoic acid early-inducible protein 1-delta
基因别名: RAE-1delta; Raet1d
UniProt ID: (Mouse) Q9JI58
Entrez Gene ID: (Mouse) 56554
