Invitrogen

RANGAP1 Polyclonal Antibody

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RANGAP1 Antibody (PA5-30143) in WB

Western blot was performed using Anti-RANGAP1 Polyclonal Antibody (Product # PA5-30143) and 63 kDa band corresponding to RANGAP1 was observed along with ~70, 80 kDa bands corresponding to SUMOylated form of RANGAP1 was observed across the cell lines tested. Whole cell Extracts of (30 µg lysate) of K-562 (Lane 1), HEK-293 (Lane 2), HeLa (Lane 3), 3T3-L1 (Lane 4) , OVCAR-3 (Lane 5),Raji (Lane 6) and Mouse Testis (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferr... View More

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产品信息

PA5-30143

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:10,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 225 of Human RanGAP1

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2547617

产品详细信息

Recommended positive controls: 293T, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat2.

Predicted reactivity: Mouse (96%), Rat (95%), Xenopus laevis (86%), Chicken (90%), Bovine (96%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs. RanGDP involved in signaling. Mammalian RanGAP is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。