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RAP80 Antibody (PA5-120277) in WB
Western blot analysis of RAP80 in extracts of various cell lines. Samples were incubated with RAP80 Polyclonal antibody (Product # PA5-120277) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
产品信息
PA5-120277
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 449-719 of human UIMC1 (NP_057374.3).
偶联物
形式
Liquid
浓度
0.52 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2913849
产品详细信息
Positive test controls include: DU145, 293T, HeLa, Mouse thymus. The target is usually found in the following locations: Nucleus.
Immunogen sequence: QLSSSETFDL EREVSPGSRD ILDGVRIIMA DKEVGNKEDA EKEVAISTFS SSNQVSCPLC DQCFPPTKIE RHAMYCNGLM EEDTVLTRRQ KEAKTKSDSG TAAQTSLDID KNEKCYLCKS LVPFREYQCH VDSCLQLAKA DQGDGPEGSG RACSTVEGKW QQRLKNPKEK GHSEGRLLSF LEQSEHKTSD ADIKSSETGA FRVPSPGMEE AGCSREMQSS FTRRDLNESP VKSFVSISEA TDCLVDFKKQ VTVQPGSRTR TKAGRGRRRK F
靶标信息
RAP80 was initially identified as zinc-finger containing nuclear protein that is highly expressed in testis and interacts with the retinoid-related testis-associated receptor (RTR). Later experiments revealed that RAP80 is recruited by the Coiled-coil domain 98 (CCDC98) protein to the breast cancer-1 protein BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both RAP80 and CCDC98 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either RAP80 or CCDC98 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that RAP80 and CCDC98 control only part of the DNA damage response role of BRCA1. At least four isoforms of RAP80 are known to exist.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: BRCA1-A complex subunit RAP80; Receptor-associated protein 80; retinoid X receptor interacting protein 110; Retinoid X receptor-interacting protein 110; Ubiquitin interaction motif-containing protein 1
基因别名: 9430016E08Rik; D330018D10Rik; D630032M02Rik; RAP80; RIP110; RXRIP110; UIMC1; X2HRIP110
UniProt ID: (Human) Q96RL1, (Mouse) Q5U5Q9
Entrez Gene ID: (Human) 51720, (Mouse) 20184
double-strand break repair
double-strand break repair via nonhomologous end joining
transcription, DNA-templated
response to ionizing radiation
G2 DNA damage checkpoint
positive regulation of DNA repair
negative regulation of transcription, DNA-templated
histone H2A K63-linked deubiquitination
DNA repair
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
chromatin modification
