Invitrogen

RNF41 Polyclonal Antibody

RNF41 Antibody in Immunocytochemistry (ICC/IF)

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RNF41 Antibody (PA5-103818) in ICC/IF

Immunocytochemistry analysis of RNF41 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RNF41 polyclonal antibody (Product # PA5-103818) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

RNF41 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-103818

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human RNF41(Accession Q9H4P4), corresponding to amino acid residues V141-E191.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

AB_2853151

产品详细信息

Antibody detects endogenous levels of total RNF41.

靶标信息

The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. Nrdp1, also known as RNF41 (RING finger protein 41), SBBI03 or FLRF, is a 317 amino acid protein that contains one RING-type zinc finger and one SIAH-type zinc finger. Expressed in testis, ovary and prostate, Nrdp1 functions as an E3 ubiquitin-protein ligase that, characteristic of E3 ligase proteins, accepts ubiquitin (in the form of a thioester) from an E2 ubiquitin-conjugating enzyme and transfers that ubiquitin residue to substrates targeted for degradation. Specifically, Nrdp1 interacts with ErbB-3 and UBPY, thereby targeting them for proteasomal degradation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: E3 ubiquitin-protein ligase NRDP1; fetal liver ring finger; neuregulin receptor degradation protein-1; RING finger protein 41; ring finger protein 41, E3 ubiquitin protein ligase; RING-type E3 ubiquitin transferase NRDP1

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基因别名: 2210404G21Rik; 4930511A05Rik; 4933415P08Rik; D10Ertd722e; FLRF; NRDP1; RNF41; SBBI03

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UniProt ID: (Human) Q9H4P4, (Mouse) Q8BH75

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Entrez Gene ID: (Human) 10193, (Mouse) 67588, (Rat) 362814

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