Invitrogen

RPL5 Polyclonal Antibody

RPL5 Antibody in Immunocytochemistry (ICC/IF)

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RPL5 Antibody (PA5-27539) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of RPL5 was performed in HepG2 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RPL5 Polyclonal Antibody (Product # PA5-27539) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

RPL5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

RPL5 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with RPL5 siRNA and reduction of signal was observed in Western Blot using RPL5 Polyclonal Antibody (Product # PA5-27539). {KD}

产品信息

PA5-27539

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化 (IHC)

- -

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

in situ PLA (PLA)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 142 and 297 of Human RPL5

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.4 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2545015

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse brain, Rat2.

Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (84%), Pig (97%), Rabbit (98%), Chicken (90%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

RPL5 encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A single synonymous mutation determines the phosphorylation and stability of the nascent protein.

Journal of molecular cell biology

Karakostis K,Vadivel Gnanasundram S,López I,Thermou A,Wang L,Nylander K,Olivares-Illana V,Fåhraeus R

PA5-27539 was used in Proximity Ligation Assay to show how the ATM kinase phosphorylates the p53 protein while it is being synthesized and offer a novel mechanism whereby a single synonymous mutation controls the stability and activity of the encoded protein.