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Ribophorin II Antibody (PA5-97018) in WB
Western blot analysis of Ribophorin II in extracts of various cell lines. Samples were incubated with Ribophorin II Polyclonal antibody (Product # PA5-97018) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
产品信息
PA5-97018
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 360-540 of human RPN2 (NP_0029422)
偶联物
形式
Liquid
浓度
0.94 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2808820
产品详细信息
Immunogen sequence: ANTVELRVKI STEVGITNVD LSTVDKDQSI APKTTRVTYP AKAKGTFIAD SHQNFALFFQ LVDVNTGAEL TPHQTFVRLH NQKTGQEVVF VAEPDNKNVY KFELDTSERK IEFDSASGTY TLYLIIGDAT LKNPILWNVA DVVIKFPEEE APSTVLSQNL FTPKQEIQHL FREPEKRPPT V; Positive Samples: HepG2, 293T, Jurkat, MCF7, NIH/3T3, Mouse liver, Rat liver; Cellular Location: Endoplasmic reticulum membrane, Multi-pass membrane protein
靶标信息
RNP2 a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2; oligosaccharyltransferase complex subunit (non-catalytic); RIBIIR; ribophorin 2; Ribophorin II; Ribophorin-2; RPN-II
基因别名: 1300012C06Rik; AV261018; RIBIIR; Rpn-2; RPN-II; RPN2; RPNII; SWP1
UniProt ID: (Human) Q9BUQ3, (Rat) P25235, (Mouse) Q9DBG6
Entrez Gene ID: (Human) 6185, (Rat) 64701, (Mouse) 20014
