Invitrogen

SOD1 Polyclonal Antibody

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SOD1 Antibody (PA5-27240) in WB

Western blot analysis was performed on whole cell extracts of HEK-293 (Lane 1), HEK-293 treated with H2O2 (10uM, 6hrs) (Lane 2), HeLa (Lane 3), MCF-7 (Lane 4), and SH-SY5Y (Lane 5). The blot was probed with SOD1 Polyclonal Antibody (Product # PA5-27240, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 18kDa band corresponding to SOD1 was observed across the panel and was upregulated upon H2O2 treatmen... View More

SOD1 Antibody in Immunohistochemistry (IHC)

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SOD1 Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Western blot analysis of SOD1 using SOD1 Polyclonal Antibody (Product # PA5-27240) shows an increase in expression upon treatment with H2O2 in HEK-293 cells. {TM}

产品信息

PA5-27240

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:10,000

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免疫组化 (IHC)

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免疫组化（冰冻） (IHC (F))

Assay-dependent

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染色质免疫沉淀实验 (ChIP)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to a region within amino acids 75 and 154 of Human SOD1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.21 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2544716

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, NT2D1, PC-3, SK-N-SH, Rat brain, U87-MG.

Predicted reactivity: Mouse (100%), Rat (100%), Chimpanzee (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

SOD1 (superoxide dismutase) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Simulated Microgravity Exposure Induces Antioxidant Barrier Deregulation and Mitochondria Enlargement in TCam-2 Cell Spheroids.

Cells

Berardini M,Gesualdi L,Morabito C,Ferranti F,Reale A,Zampieri M,Karpach K,Tinari A,Bertuccini L,Guarnieri S,Catizone A,Mariggiò MA,Ricci G

PA5-27240 was used in Western Blotting to demonstrate TCam-2 spheroid sensitivity to acute s-microgravity exposure and indicate the capability of these cells to trigger compensatory mechanisms that allow them to overcome the exposure to altered gravitational force.

Sat Aug 19 00:00:00 UTC 2023

Simulated Microgravity Exposure Induces Antioxidant Barrier Deregulation and Mitochondria Enlargement in TCam-2 Cell Spheroids.

Cells

Berardini M,Gesualdi L,Morabito C,Ferranti F,Reale A,Zampieri M,Karpach K,Tinari A,Bertuccini L,Guarnieri S,Catizone A,Mariggiò MA,Ricci G

Sat Aug 19 00:00:00 UTC 2023

Chronic oxidative stress adaptation in head and neck cancer cells generates slow-cyclers with decreased tumour growth in vivo.

British journal of cancer

Berner J,Miebach L,Kordt M,Seebauer C,Schmidt A,Lalk M,Vollmar B,Metelmann HR,Bekeschus S

PA5-27240 was used in Simple Western - Size to show that combination treatments together with cetuximab, an EGFR inhibitor, may overcome acquired oxidative stress resistance in HNC.

Fri Sep 01 00:00:00 UTC 2023

Preventive Effect of Limosilactobacillus fermentum SCHY34 on Lead Acetate-Induced Neurological Damage in SD Rats.

Frontiers in nutrition

Long X,Wu H,Zhou Y,Wan Y,Kan X,Gong J,Zhao X

PA5-27240 was used in Western Blotting to show that L. fermentum SCHY34 showed a great biological scavenging and potential effect on alleviating the toxicity of lead ions.

Sat Jul 16 00:00:00 UTC 2022

Expression of antioxidant enzymes in lesions of multiple sclerosis and its models.

Scientific reports

Moezzi D,Dong Y,Jain RW,Lozinski BM,Ghorbani S,D'Mello C,Wee Yong V

PA5-27240 was used in Immunohistochemistry-immunofluorescence to investigate whether antioxidant enzyme elevation in mouse models of neurodegeneration corresponds to their expression in human diseases such as multiple sclerosis.

Tue Jul 26 00:00:00 UTC 2022

Expression of antioxidant enzymes in lesions of multiple sclerosis and its models.

Scientific reports

Moezzi D,Dong Y,Jain RW,Lozinski BM,Ghorbani S,D'Mello C,Wee Yong V

Tue Jul 26 00:00:00 UTC 2022

Hatched Eggshell Membrane Can Be a Novel Source of Antioxidant Hydrolysates to Protect against H₂O₂-Induced Oxidative Stress in Human Chondrocytes.

Antioxidants (Basel, Switzerland)

Zhu L,Ma M,Ahn DU,Guyonnet V,Wang L,Zheng Y,He Q,Xiong H,Huang X

PA5-27240 was used in Immunohistochemistry (Frozen), Western Blot to provide a theoretical basis for the value-added application of hatched ESM waste to produce antioxidant hydrolysates and indicates their potential as functional food and pharmaceuticals.

Fri Dec 09 00:00:00 UTC 2022

The Transcriptional Complex Sp1/KMT2A by Up-Regulating Restrictive Element 1 Silencing Transcription Factor Accelerates Methylmercury-Induced Cell Death in Motor Neuron-Like NSC34 Cells Overexpressing SOD1-G93A.

Frontiers in neuroscience

Guida N,Sanguigno L,Mascolo L,Calabrese L,Serani A,Molinaro P,Lau CG,Annunziato L,Formisano L

PA5-27240 was used in ChIP assay to demonstrate that MeHg hastens necroptotic cell death in SOD1-G93A cells via Sp1/KMT2A complex, that by epigenetic mechanisms increases REST gene expression.

Wed Dec 15 00:00:00 UTC 2021

Frontiers in neuroscience

Guida N,Sanguigno L,Mascolo L,Calabrese L,Serani A,Molinaro P,Lau CG,Annunziato L,Formisano L

PA5-27240 was used in ChIP assay to demonstrate that MeHg hastens necroptotic cell death in SOD1-G93A cells via Sp1/KMT2A complex, that by epigenetic mechanisms increases REST gene expression.

Wed Dec 15 00:00:00 UTC 2021

Resveratrol treatment reduces the vulnerability of SH-SY5Y cells and cortical neurons overexpressing SOD1-G93A to Thimerosal toxicity through SIRT1/DREAM/PDYN pathway.

Neurotoxicology

Laudati G,Mascolo L,Guida N,Sirabella R,Pizzorusso V,Bruzzaniti S,Serani A,Di Renzo G,Canzoniero LMT,Formisano L

PA5-27240 was used in Western Blot to demonstrate the particular vulnerability of SOD1-G93 A neuronal cells to thimerosal and that RSV via SIRT1 counteracts the neurodetrimental effect of this toxicant by preventing DREAM reduction and prodynorphin up-regulation.

Fri Mar 01 00:00:00 UTC 2019

Oxidative stress and mitochondrial uncoupling protein 2 expression in hepatic steatosis induced by exposure to xenobiotic DDE and high fat diet in male Wistar rats.

PloS one

Migliaccio V,Scudiero R,Sica R,Lionetti L,Putti R

PA5-27240 was used in Western Blotting to aimed to evaluate hepatic oxidative stress and anti-oxidant systems response in rats exposed to HFD and/or non-toxic dose of dichlorodiphenyldichloroethylene.

Wed Jan 08 00:00:00 UTC 2020

Methylmercury alters proliferation, migration, and antioxidant capacity in human HTR8/SV-neo trophoblast cells.

Reproductive toxicology (Elmsford, N.Y.)

Tucker EK,Nowak RA

PA5-27240 was used in Western Blotting to indicate that trophoblast cells react differently to methylmercury relative to neural cell lines, and thus investigation of methylmercury toxicity in placental cells is needed to understand the effects of this heavy metal on the placenta.

Fri Jun 01 00:00:00 UTC 2018

In vivo genome editing improves motor function and extends survival in a mouse model of ALS.

Science advances

Gaj T,Ojala DS,Ekman FK,Byrne LC,Limsirichai P,Schaffer DV

PA5-27240 was used in Western Blotting to demonstrate that CRISPR-Cas9 can be harnessed to disrupt mutant SOD1 expression in the G93ASOD1 mouse model of Amyotrophic lateral sclerosis (ALS) following in vivo delivery using an adeno-associated virus vector.

Fri Dec 01 00:00:00 UTC 2017

生物信息学

蛋白别名: Amyotrophic lateral sclerosis 1 (ALS1); Cu(2+)-Zn2+ superoxide dismutase; Cu/Zn SOD; Cu/Zn superoxide dismutase; epididymis secretory protein Li 44; HGNC:11179; hSod1; indophenoloxidase A; Indophenoloxidase A (IPOA); SOD, soluble; superoxide dismutase; Superoxide dismutase 1; Superoxide Dismutase 1 (SOD1); Superoxide dismutase [Cu-Zn]; superoxide dismutase, cystolic

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基因别名: ALS; ALS1; B430204E11Rik; Cu/Zn-SOD; CuZnSOD; HEL-S-44; homodimer; hSod1; Ipo-1; Ipo1; IPOA; SOD; Sod-1; SOD1; SODC

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UniProt ID: (Human) P00441, (Mouse) P08228, (Rat) P07632

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Entrez Gene ID: (Human) 6647, (Mouse) 20655, (Rat) 24786

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功能

superoxide dismutase activity copper ion binding protein binding zinc ion binding protein phosphatase 2B binding identical protein binding protein homodimerization activity Rac GTPase binding chaperone binding antioxidant activity oxidoreductase activity enzyme binding metal ion binding oxidoreductase

参与通路

activation of MAPK activity response to reactive oxygen species response to superoxide ovarian follicle development positive regulation of cytokine production placenta development retina homeostasis response to amphetamine myeloid cell homeostasis platelet degranulation glutathione metabolic process superoxide metabolic process cellular iron ion homeostasis spermatogenesis embryo implantation cell aging sensory perception of sound locomotory behavior anterograde axonal transport retrograde axonal transport regulation of blood pressure response to heat response to organic substance transmission of nerve impulse removal of superoxide radicals response to nutrient levels peripheral nervous system myelin maintenance positive regulation of superoxide anion generation regulation of T cell differentiation in thymus response to carbon monoxide cellular response to potassium ion regulation of multicellular organism growth response to drug response to hydrogen peroxide superoxide anion generation positive regulation of apoptotic process positive regulation of catalytic activity regulation of GTPase activity negative regulation of neuron apoptotic process response to ethanol negative regulation of cholesterol biosynthetic process regulation of protein kinase activity regulation of organ growth response to antibiotic response to copper ion muscle cell cellular homeostasis thymus development response to axon injury hydrogen peroxide biosynthetic process regulation of mitochondrial membrane potential oxidation-reduction process heart contraction neurofilament cytoskeleton organization relaxation of vascular smooth muscle auditory receptor cell stereocilium organization cellular response to cadmium ion cellular response to ATP reactive oxygen species metabolic process response to antipsychotic drug positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway response to oxidative stress aging negative regulation of apoptotic process double-strand break repair apoptotic DNA fragmentation cellular response to oxidative stress

SOD1 Polyclonal Antibody

SOD1 Antibody (PA5-27240) in WB

产品信息

PA5-27240

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（冰冻） (IHC (F))

染色质免疫沉淀实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

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SOD1 Polyclonal Antibody

SOD1 Antibody (PA5-27240) in WB

产品信息

PA5-27240

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（冰冻） (IHC (F))

染色质免疫沉淀 实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)