Invitrogen

SPI-B Monoclonal Antibody (235D), eBioscience™

SPI-B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SPI-B Antibody (14-9790-82) in IHC (P)

Immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue using 5 µg/mL of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (left) or 5 µg/mL of Anti-Human SPI-B Purified (right) followed by Anti-Mouse Biotin, Streptavidin-HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.

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产品信息

14-9790-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-Dependent

免疫组化（石蜡） (IHC (P))

5 µg/mL

免疫细胞化学 (ICC/IF)

Assay-Dependent

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

235D

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2573035

产品详细信息

Description: The 235D monoclonal antibody recognizes human SPI-B. SPI-B is a member of the ETS transcription factor family which is comprised of DNA binding proteins that function in lymphoid development and activity. SPI-B binds the PU box, a purine-rich DNA sequence (5'-GAGGAA-3'). SPI-B is expressed in mature B cells, immature T cells, plasmacytoid dendritic cells, and microfold cells (M cells) of the intestine. In human tonsil tissue, high levels of SPI-B are expressed in plasmacytoid dendritic cells, while lower levels of expression are found in germinal center B cells.

Applications Reported: This 235D antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, immunohistochemical staining, and immunocytochemistry.

Applications Tested: This 235D antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Spi-B is a transcription factor belonging to a subfamily of ETS transcription factors with a conserved ETS domain that mediates specific DNA binding. Spi-B may form homotypic interactions and is known to interact with IRF4, CREBBP, EP300, SPI1/PU.1 related, JUN and TBP. As a sequence specific transcriptional activator it binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Two isoforms of Spi-B are reported. Spi-B is usually expressed in plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells and promotes their development and block viral replication. Spi-B is not expressed in T-cells or granulocytes but may be present in stem cell populations of the liver and B-cells where it helps in B-cell receptor (BCR) signaling, normal B-cell development and antigenic stimulation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。