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STAT1 Antibody (MA5-15129) in ICC/IF
Immunofluorescence analysis of Signal transducer and activator of transcription 1-alpha/beta was performed using 70% confluent log phase HeLa 50 ng/mL IFN gamma treatment for 30 min. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with STAT1 Monoclonal Antibody (C.156.9) (Product # MA5-15129) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabb... View More
产品信息
MA5-15129
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Non-human primate
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
C.156.9
抗原
Synthetic peptide corresponding to the sequence of human Stat1
偶联物
形式
Liquid
浓度
167 µg/mL
纯化类型
Affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物
<0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_10984833
产品详细信息
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
It is not recommended to aliquot this antibody.
靶标信息
STAT1 (signal transducers and activators of transcription 1) is a member of the STAT family of transcription factors. STAT1 can be activated by interferon-alpha, interferon-gamma, EGF, PDGF and IL6. STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. Further, STAT1 plays an important role in mediating cell viability in response to different cell stimuli and pathogen exposure. The STAT1 gene is located on chromosome 2. STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes. Among the seven STATs types, STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-Alpha, IFN-Beta, IFN-gamma and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DKFZp686B04100; OTTHUMP00000165046; OTTHUMP00000205845; signal transducer and activator of transcription 1, 91kD; signal transducer and activator of transcription 1, 91kDa; Signal transducer and activator of transcription 1-alpha/beta; signal transducer and activator of transcription-1; STAT; STAT 1; STAT-1; stat1 alpha; Transcription factor ISGF-3 components p91/p84
基因别名: CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT1; STAT91
UniProt ID: (Human) P42224
Entrez Gene ID: (Human) 6772
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter sequence-specific
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
signal transducer activity
tumor necrosis factor receptor binding
protein binding
enzyme binding
nuclear hormone receptor binding
identical protein binding
protein homodimerization activity
cadherin binding involved in cell-cell adhesion
DNA-binding transcription factor
negative regulation of transcription from RNA polymerase II promoter
negative regulation of endothelial cell proliferation
positive regulation of mesenchymal cell proliferation
negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
transcription, DNA-templated
apoptotic process
JAK-STAT cascade
blood circulation
macrophage derived foam cell differentiation
negative regulation of angiogenesis
tumor necrosis factor-mediated signaling pathway
response to cytokine
response to interferon-beta
cellular response to interferon-beta
regulation of apoptotic process
negative regulation of I-kappaB kinase/NF-kappaB signaling
response to peptide hormone
endothelial cell migration
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
negative regulation by virus of viral protein levels in host cell
positive regulation of smooth muscle cell proliferation
response to cAMP
defense response to virus
interferon-gamma-mediated signaling pathway
regulation of interferon-gamma-mediated signaling pathway
type I interferon signaling pathway
regulation of type I interferon-mediated signaling pathway
renal tubule development
cellular response to organic cyclic compound
metanephric mesenchymal cell proliferation involved in metanephros development
metanephric mesenchymal cell differentiation
negative regulation of metanephric nephron tubule epithelial cell differentiation
cell-cell adhesion
