Invitrogen

STING Recombinant Rabbit Monoclonal Antibody (2H1L5)

STING Antibody in Immunocytochemistry (ICC/IF)

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STING Antibody (702993) in ICC/IF

Immunofluorescence of STING1 was performed using PMA-treated THP-1 WT and STING1 KO cells that were labeled with a green or a far-red fluorescent dye, respectively. Post-labeling, WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with an optically clear flat bottom as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min at RT; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS containing 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min at RT. Cell... View More

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STING Antibody — Altered expression of the protein upon cell treatment demonstrates antibody specificity. Western blot using Anti-STING Recombinant Rabbit Monoclonal Antibody (Product # 702993, 1:5000 dilution), shows increased expression of protein STING in THP-1 cells upon treatment with 2’3’-cGAMP. {TM}

产品信息

702993

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000

免疫细胞化学 (ICC/IF)

1:250

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

2H1L5

抗原

Protein corresponding to human STING [aa139-aa379]

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2762404

产品详细信息

This antibody is predicted to react with Monkey, Goat, Sheep.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

STING is a 379 amino acid containing signaling protein that acts as an important component of innate immune signaling. This signaling protein is able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon (IFN-alpha and IFN-beta) and exert a potent anti-viral state following expression. STING may have translocon function, the translocon possibly being able to influence the induction of type I interferons and is also involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II) thus facilitating the detection of intracellular viral RNA species as well as B-form DNA and mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway. Generally seen in endoplasmic reticulum membrane, cell membrane and mitochondrion outer membrane, it is ubiquitously expressed in most of tissues.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: endoplasmic reticulum IFN stimulator; Endoplasmic reticulum interferon stimulator; ERIS; hMITA; hSTING; Mediator of IRF3 activation; mitochondrial mediator of IRF3 activation; N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner; Stimulator of interferon genes protein; tm173; Transmembrane protein 173

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基因别名: ERIS; hMITA; hSTING; MITA; MPYS; NET23; SAVI; STING; STING1; TMEM173

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UniProt ID: (Human) Q86WV6

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Entrez Gene ID: (Human) 340061

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功能

protein binding transcription factor binding protein kinase binding ubiquitin protein ligase binding cyclic-di-GMP binding identical protein binding protein homodimerization activity cyclic-GMP-AMP binding

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