Invitrogen

STRAP Monoclonal Antibody (A7H11)

STRAP Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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STRAP Antibody (MA5-50560) in IHC (P)

Immunohistochemistry of STRAP in paraffin-embedded human testis tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with STRAP monoclonal antibody (Product # MA5-50560) with a dilution of 1:200 for 1 hour at room temperatur... View More

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产品信息

MA5-50560

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

免疫组化（石蜡） (IHC (P))

1:200

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

A7H11

抗原

Recombinant protein within human STRAP aa 201-350/350.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_3091211

产品详细信息

Positive control: 293T cell lysate, Jurkat cell lysate, A549 cell lysate, MCF-7 cell lysate, SH-SY5Y cell lysate, human testis tissue.

Predicted band size: 38 kDa

Subcellular Location: Cytoplasm, Nucleus.

靶标信息

STRAP is a 350 amino acid MAP activator with WD-40 repeats that is localized both in the cytoplasm and the nucleus. It is a ubiquitously expressed protein that acts as an inhibitor of TGF-beta signaling and an important regulator of cell proliferation. Stable expression of STRAP results in activation of the mitogen-activated protein kinase/extracellular signal-regulated kinase pathway and in down-regulation of the cyclin-dependent kinase inhibitor p21 (Cip1), which results in retinoblastoma protein hyperphosphorylation. Upregulation of STRAP might play a role in tumor development as an early event for colorectal cancers. It is the first component of the U snRNP assembly machinery that associates with SMN complex in a compartment-specific way and plays a crucial role in the intracellular distribution of SMN1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。