Invitrogen

Snail1 Monoclonal Antibody (20C8), eBioscience™

Snail1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Snail1 Antibody (14-9859-82) in IHC (P)

Immunohistochemistry on formalin-fixed paraffin embedded human infiltating ductal carcinoma using 5 µg/mL Mouse IgG2a Isotype Control (left) or 5 µg/mL Anti-Human Snail1 Purified (right) followed by anti-mouse IgG HRP and DAB visualization.Nuclei are counterstained with hematoxylin.

Snail1 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)

Snail1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Snail1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

Snail1 Antibody in Immunocytochemistry (ICC/IF)

Snail1 Antibody in Western Blot, Immunohistochemistry, Immunoprecipitation (WB, IHC, IP)

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产品信息

14-9859-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

查看9篇已发表文章 9篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

5 µg/mL

免疫细胞化学 (ICC/IF)

Assay-Dependent

查看4篇已发表文章 4篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-Dependent

免疫沉淀 (IP)

Assay-Dependent

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

20C8

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_2573045

产品详细信息

Description: The monoclonal antibody 20C8 recognizes human Snail1, a 29 kDa zinc finger transcription factor. Snail1 and Slug both belong to a family of transcription factors that are responsible for inducing epithelial to mesenchymal transition (EMT) during normal organ development and tumor progression. Snail1 expression is localized to both the cytoplasm and nucleus and functions as a transcriptional repressor of E-cadherin, claudins, occludin, and desmoplakin. During embryonic development, Snail1 is localized to the mesoderm, while in the adult, expression is inducible and is usually limited to activated fibroblasts and some mesenchymal cells. Overexpression of Snail1 has been found in carcinomas and fibrosarcomas and may be used as an early marker for EMT.

Applications Reported: This 20C8 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded (IHC-P) tissue, ELISA, immunoprecipitation, western blotting, and immunocytochemistry.

Applications Tested: This 20C8 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded (FFPE) human breast cancer tissue using low pH antigen retrieval. The antibody can also be used following high pH antigen retrieval. This antibody can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Zinc finger protein SNAI1 (SNAIL)is a zinc finger transcriptional repressor which down regulates the expression of the ectodermal gene within the mesoderm and functions to form the mesoderm and neural crest. It is reported that SNAI1 acts as an important factor of tumor invasion as evidenced by its role in E-cadherin down-regulation and induction of epithelial-mesenchymal transition (EMT). In human breast cancer, the expression of SNAI1 and/or the homologous SNAI2 (Slug) has been associated with E-cadherin repression, local or distant metastasis, tumor recurrence, or poor prognosis in different tumor series. It is also reported that Snail 1 protein in the stromamay be anew putative prognosis marker for colon tumors.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Epigenetic upregulation of MNAT1 by SMYD2 is linked to PI3K/AKT activation and tumorigenesis of pancreatic adenocarcinoma.

Histology and histopathology

Xu Z,Liu Y,Pan Z,Qin L

14-9859-82 was used in Western Blot to investigate the roles of SMYD2 and its interacted molecules in pancreatic adenocarcinoma (PAAD).

Thu Feb 01 00:00:00 UTC 2024

Lactate promotes endothelial-to-mesenchymal transition via Snail1 lactylation after myocardial infarction.

Science advances

Fan M,Yang K,Wang X,Chen L,Gill PS,Ha T,Liu L,Lewis NH,Williams DL,Li C

14-9859-82 was used in Immunohistochemistry to conclude that lactate acts as an important molecule that up-regulates cardiac.

Fri Feb 03 00:00:00 UTC 2023

Lactate promotes endothelial-to-mesenchymal transition via Snail1 lactylation after myocardial infarction.

Science advances

Fan M,Yang K,Wang X,Chen L,Gill PS,Ha T,Liu L,Lewis NH,Williams DL,Li C

14-9859-82 was used in Immunohistochemistry to conclude that lactate acts as an important molecule that up-regulates cardiac.

Fri Feb 03 00:00:00 UTC 2023

Lactate promotes endothelial-to-mesenchymal transition via Snail1 lactylation after myocardial infarction.

Science advances

Fan M,Yang K,Wang X,Chen L,Gill PS,Ha T,Liu L,Lewis NH,Williams DL,Li C

14-9859-82 was used in Immunohistochemistry to conclude that lactate acts as an important molecule that up-regulates cardiac.

Fri Feb 03 00:00:00 UTC 2023

Lactate promotes endothelial-to-mesenchymal transition via Snail1 lactylation after myocardial infarction.

Science advances

Fan M,Yang K,Wang X,Chen L,Gill PS,Ha T,Liu L,Lewis NH,Williams DL,Li C

14-9859-82 was used in Immunohistochemistry to conclude that lactate acts as an important molecule that up-regulates cardiac.

Fri Feb 03 00:00:00 UTC 2023

Aberrant Akt2 signaling in the RPE may contribute to retinal fibrosis process in diabetic retinopathy.

Cell death discovery

Daley R,Maddipatla V,Ghosh S,Chowdhury O,Hose S,Zigler JS,Sinha D,Liu H

14-9859-82 was used in Western Blot to find that in Akt2 cKO mice diabetes-induced increase of fibrosis markers, including collagen IV, Connective tissue growth factor (CTGF), fibronectin, and alpha-SMA was attenuated.

Thu Jul 13 00:00:00 UTC 2023

PRR15 deficiency facilitates malignant progression by mediating PI3K/Akt signaling and predicts clinical prognosis in triple-negative rather than non-triple-negative breast cancer.

Cell death & disease

Guo F,Ma J,Li C,Liu S,Wu W,Li C,Wang J,Wang J,Li Z,Zhai J,Sun F,Zhou Y,Guo C,Qian H,Xu B

Published figure using Snail1 monoclonal antibody (Product # 14-9859-82) in Western Blot

Tue Apr 18 00:00:00 UTC 2023

Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3β/Tyr216 Accumulation.

Journal of oncology

He Z,Lei S,Liang F,Tan L,Zhang W,Xie L,Zheng H,Lu Y

Published figure using Snail1 monoclonal antibody (Product # 14-9859-82) in Western Blot

Fri Dec 31 00:00:00 UTC 2021

Circular RNA_0000629 Suppresses Bladder Cancer Progression Mediating MicroRNA-1290/CDC73.

Cancer management and research

Wang J,Luo J,Wu X,Gao Z

14-9859-82 was used in Western Blot to demonstrate that Circ_0000629 promotes the expression of CDC73 by competitively binding to miR-1290, thereby inhibiting the growth and metastasis of BC cells.

Thu Apr 21 00:00:00 UTC 2022

MiR-195 inhibits the ubiquitination and degradation of YY1 by Smurf2, and induces EMT and cell permeability of retinal pigment epithelial cells.

Cell death & disease

Fu SH,Lai MC,Zheng YY,Sun YW,Qiu JJ,Gui F,Zhang Q,Liu F

Published figure using Snail1 monoclonal antibody (Product # 14-9859-82) in Immunocytochemistry

Thu Jul 15 00:00:00 UTC 2021

MiR-195 inhibits the ubiquitination and degradation of YY1 by Smurf2, and induces EMT and cell permeability of retinal pigment epithelial cells.

Cell death & disease

Fu SH,Lai MC,Zheng YY,Sun YW,Qiu JJ,Gui F,Zhang Q,Liu F

Published figure using Snail1 monoclonal antibody (Product # 14-9859-82) in Immunocytochemistry

Thu Jul 15 00:00:00 UTC 2021

EIF3H promotes aggressiveness of esophageal squamous cell carcinoma by modulating Snail stability.

Journal of experimental & clinical cancer research : CR

Guo X,Zhu R,Luo A,Zhou H,Ding F,Yang H,Liu Z

14-9859 was used in Immunoprecipitation to reveal a critical EIF3H-Snail signaling axis in tumor aggressiveness in ESCC and provides EIF3H as a promising biomarker for ESCC treatment.

Mon Aug 31 00:00:00 UTC 2020

EIF3H promotes aggressiveness of esophageal squamous cell carcinoma by modulating Snail stability.

Journal of experimental & clinical cancer research : CR

Guo X,Zhu R,Luo A,Zhou H,Ding F,Yang H,Liu Z

14-9859 was used in Immunoprecipitation to reveal a critical EIF3H-Snail signaling axis in tumor aggressiveness in ESCC and provides EIF3H as a promising biomarker for ESCC treatment.

Mon Aug 31 00:00:00 UTC 2020

EIF3H promotes aggressiveness of esophageal squamous cell carcinoma by modulating Snail stability.

Journal of experimental & clinical cancer research : CR

Guo X,Zhu R,Luo A,Zhou H,Ding F,Yang H,Liu Z

14-9859 was used in Immunoprecipitation to reveal a critical EIF3H-Snail signaling axis in tumor aggressiveness in ESCC and provides EIF3H as a promising biomarker for ESCC treatment.

Mon Aug 31 00:00:00 UTC 2020

HOXC10 promotes migration and invasion via the WNT-EMT signaling pathway in oral squamous cell carcinoma.

Journal of Cancer

Dai BW,Yang ZM,Deng P,Chen YR,He ZJ,Yang X,Zhang S,Wu HJ,Ren ZH

Published figure using Snail1 monoclonal antibody (Product # 14-9859-82) in Immunofluorescence

Wed Sep 30 00:00:00 UTC 2020

PADI4‑mediated epithelial‑mesenchymal transition in lung cancer cells.

Molecular medicine reports

Liu M,Qu Y,Teng X,Xing Y,Li D,Li C,Cai L

14-9859-82 was used in Western Blot to evaluate the expression of protein‚arginine deiminase type‚4 and its associated molecular signalling in lung cancer metastasis.

Mon Apr 01 00:00:00 UTC 2019

A Role for βA3/A1-Crystallin in Type 2 EMT of RPE Cells Occurring in Dry Age-Related Macular Degeneration.

Investigative ophthalmology & visual science

Ghosh S,Shang P,Terasaki H,Stepicheva N,Hose S,Yazdankhah M,Weiss J,Sakamoto T,Bhutto IA,Xia S,Zigler JS,Kannan R,Qian J,Handa JT,Sinha D

14-9859-82 was used in Western Blot to study the role of βA3/A1-crystallin in age related macular degeneration.

Tue Mar 20 00:00:00 UTC 2018

生物信息学

蛋白别名: Protein sna; Protein snail homolog 1; Sna protein; snai; snail 1; snail 1 homolog; snail 1 zinc finger protein; snail 1, zinc finger protein; snail family zinc finger 1; snail homolog 1; Zinc finger protein SNAI1

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基因别名: dJ710H13.1; SLUGH2; SNA; SNAH; SNAI1; SNAIL; SNAIL1

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UniProt ID: (Human) O95863

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Entrez Gene ID: (Human) 6615

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功能

RNA polymerase II regulatory region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding protein binding kinase binding metal ion binding C2H2 zinc finger transcription factor

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