Invitrogen

Syndecan 2 Polyclonal Antibody

Syndecan 2 Antibody in Immunocytochemistry (ICC/IF)

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Syndecan 2 Antibody (36-6200) in ICC/IF

Immunofluorescence analysis of Syndecan-2 was done on 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Syndecan-2 Rabbit polyclonal Antibody (Product # 36-6200) at 2 µg/mL in 1% BSA and incubated for 3 hours at room temp... View More

Syndecan 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Syndecan 2 Antibody in Western Blot (WB)

Syndecan 2 Antibody in Immunohistochemistry (IHC)

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产品信息

36-6200

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:10-1:100

免疫细胞化学 (ICC/IF)

1:100-1:500

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流式细胞分析 (Flow)

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酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Human

已发表种属

Hamster, Human, Mammal, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from the internal region of human syndecan-2 (fibroglycan).

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533270

靶标信息

Syndecans are heparan sulfate proteoglycans (HSPGs) that are involved in cell adhesion, tissue morphogenesis, differentiation, and act as low affinity co-receptors of soluble growth factors. It is involved in the mitogenic activity and signaling of Granulocyte-Macrophage Colony-stimulating factor in osteoblasts, and has been shown to have a regulatory role in matrix assembly. Syndecan-2 is expressed at epithelial-mesenchymal interfaces, and prechondrogenic and preosteogenic mesenchymal condensations, which are areas of high morphogenetic activity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Plasma membrane proteoglycans syndecan-2 and syndecan-4 engage with EGFR and RON kinase to sustain carcinoma cell cycle progression.

The Journal of biological chemistry

Beauvais DM,Nelson SE,Adams KM,Stueven NA,Jung O,Rapraeger AC

36-6200 was used in Flow Cytometry to add to the growing list of receptor tyrosine kinases that support tumorigenesis when activated by their association with syndecans at sites of matrix adhesion and identify new potential targets for cancer therapy.

Wed Jun 01 00:00:00 UTC 2022

Osteoblastic heparan sulfate glycosaminoglycans control bone remodeling by regulating Wnt signaling and the crosstalk between bone surface and marrow cells.

Cell death & disease

Mansouri R,Jouan Y,Hay E,Blin-Wakkach C,Frain M,Ostertag A,Le Henaff C,Marty C,Geoffroy V,Marie PJ,Cohen-Solal M,Modrowski D

Published figure using Syndecan 2 polyclonal antibody (Product # 36-6200) in Immunohistochemistry

Thu Jun 29 00:00:00 UTC 2017

Heparan sulfate proteoglycans (HSPGs) and chondroitin sulfate proteoglycans (CSPGs) function as endocytic receptors for an internalizing anti-nucleic acid antibody.

Scientific reports

Park H,Kim M,Kim HJ,Lee Y,Seo Y,Pham CD,Lee J,Byun SJ,Kwon MH

36-6200 was used in Western Blotting to show that anti-DNA antibodies enter cells via both heparan sulfate proteoglycans and chondroitin sulfate proteoglycans simultaneously.

Mon Oct 30 00:00:00 UTC 2017

Syndecan-2 is a key regulator of transforming growth factor beta 2/Smad2-mediated adhesion in fibrosarcoma cells.

IUBMB life

Mytilinaiou M,Bano A,Nikitovic D,Berdiaki A,Voudouri K,Kalogeraki A,Karamanos NK,Tzanakakis GN

36-6200 was used in western blot to test if syndecan-2 affects TGFβ2 functions.

Fri Feb 01 00:00:00 UTC 2013

Differences in heparan sulfate production in cervical fibroblast cultures from women undergoing term and preterm delivery.

Acta obstetricia et gynecologica Scandinavica

Akerud A,Dubicke A,Sennstrom M,Ekman-Ordeberg G,Malmstrom A

36-6200 was used in immunocytochemistry to determine if pregnancy alters proteoglycan production in cervical fibroblasts

Mon Dec 29 00:00:00 UTC 2008

CCN2 (connective tissue growth factor) promotes fibroblast adhesion to fibronectin.

Molecular biology of the cell

Chen Y,Abraham DJ,Shi-Wen X,Pearson JD,Black CM,Lyons KM,Leask A

Wed Dec 01 00:00:00 UTC 2004

CCN2 (connective tissue growth factor) promotes fibroblast adhesion to fibronectin.

Molecular biology of the cell

Chen Y,Abraham DJ,Shi-Wen X,Pearson JD,Black CM,Lyons KM,Leask A

Wed Dec 01 00:00:00 UTC 2004

生物信息学

蛋白别名: CD362; cell surface-associated heparan sulfate proteoglycan 1; Fibroglycan; heparan sulfate proteoglycan 1, cell surface-associated; Heparan sulfate proteoglycan core protein; HSPG; SYND2; syndecan proteoglycan 2; Syndecan-2

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基因别名: CD362; HSPG; HSPG1; SDC2; SYND2

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UniProt ID: (Human) P34741

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Entrez Gene ID: (Human) 6383

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功能

protein binding PDZ domain binding transmembrane signal receptor

参与通路