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TCF7 Antibody (PA5-78191) in ICC/IF
Immunofluorescence analysis of TCF7 was performed using MOLT-4 and U-2 OS cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TCF7 Polyclonal Antibody (Product # PA5-78191) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:2... View More
产品信息
PA5-78191
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the center region of human TCF7. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2736690
产品详细信息
Positive Control: HepG2, Jurkat
Predicted Reactivity: Mouse (97%), Bovine (95%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
TCF7 (transcriptional factor 7) is a transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4+ and CD8+ immature thymocytes. Isoforms for TCF7 lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. TCF7 binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. It may also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3, and TLE4 repress transactivation mediated by TCF7 and CTNNB1.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: HMG-box; OTTHUMP00000159390; OTTHUMP00000159391; T cell factor-1; T-cell factor 1; T-cell specific; T-cell-specific transcription factor 1; TCF-7; Transcription factor 7; transcription factor 7, T-cell specific
基因别名: AI465550; TCF-1; TCF1; TCF7
UniProt ID: (Human) P36402, (Mouse) Q00417
Entrez Gene ID: (Human) 6932, (Rat) 363595, (Mouse) 21414
core promoter binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
protein binding
beta-catenin binding
sequence-specific DNA binding
transcription regulatory region DNA binding
molecular_function
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
DNA-binding transcription factor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
immune response
neural tube development
embryonic genitalia morphogenesis
T cell receptor V(D)J recombination
regulation of cell proliferation
canonical Wnt signaling pathway involved in negative regulation of apoptotic process
alpha-beta T cell differentiation
embryonic digestive tract morphogenesis
embryonic hindgut morphogenesis
canonical Wnt signaling pathway
cellular response to interleukin-4
beta-catenin-TCF complex assembly
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
brain development
biological_process
Wnt signaling pathway
generation of neurons
