Invitrogen

TIM-1 Polyclonal Antibody

TIM-1 Antibody in Immunocytochemistry (ICC/IF)

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TIM-1 Antibody (PA5-20244) in ICC/IF

Immunofluorescence analysis of TIM-1 was performed using 70% confluent log phase ACHN cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TIM-1 Polyclonal Antibody (Product # PA5-20244) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A327... View More

TIM-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TIM-1 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

TIM-1 Antibody in Immunohistochemistry (IHC)

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TIM-1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-TIM-1 Polyclonal Antibody (Product # PA5-20244), shows expression of the target protein in ACHN as compared to HeLa. {RE}

产品信息

PA5-20244

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-8 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

10 µg/mL

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免疫细胞化学 (ICC/IF)

1:50

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A 16 amino acid peptide from near the amino terminus of human TIM-1.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11152868

产品详细信息

A suggested positive control is human uterus tissue lysate.

PA5-20244 can be used with blocking peptide PEP-0363.

The PA5-20244 immunogen is located within amino acids 50 - 100 of TIM-1.

Predicted molecular ~ 44kD. In Western blot applications, this antibody has been observed to detect a band at: 60kD (Post-modification: 4 N-linked glycosylation)

Predicted species reactivity based on immunogen sequence: Mouse: (63%), Rat: (56%)

靶标信息

CD365 also known as T-cell immunoglobulin and mucin domain 1 (Tim-1) is a type 1 surface glycoprotein that consists of extracellular N-terminal immunoglobulin V-like domain and a mucin domain, and a short cytoplasmic tail. The endogenous ligand for CD365 is Tim-4. In addition, CD365 is known to be a cellular receptor or co-receptor for a number of viruses including hepatitis A and C viruses, Ebola virus, Marburg virus, Dengue virus, Zika virus, West Nile virus, and Japanese encephalitis virus. The direct binding of the viruses by CD365 occurs via phosphatildyl-serine exposed on viral envelopes. Polymorphisms within human CD365 gene (HAVCR1) are associated with susceptibility to infection and the disease severity. CD365 has also been implicated in the T cell co-stimulation. CD365 is primarily expressed in epithelial cells. It is also highly expressed in A549, 769-P and Caco-2 and cell lines. CD365 exists also as a soluble protein generated by metalloproteinase mediated proteolytic cleavage of the surface protein.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A clinically-relevant mouse model that displays hemorrhage exacerbates tourniquet-induced acute kidney injury.

Frontiers in physiology

Packialakshmi B,Burmeister DM,Anderson JA,Morgan J,Cannon G,Kiang JG,Feng Y,Lee S,Stewart IJ,Zhou X

PA5-20244 was used in Immunohistochemistry to develop a new TILLIR model that included hemorrhage and analyze the subsequent impact on kidney, lung and liver injuries.

Fri Dec 01 00:00:00 UTC 2023

Increased Endocytosis of Cadmium-Metallothionein through the 24p3 Receptor in an In Vivo Model with Reduced Proximal Tubular Activity.

International journal of molecular sciences

Zavala-Guevara IP,Ortega-Romero MS,Narváez-Morales J,Jacobo-Estrada TL,Lee WK,Arreola-Mendoza L,Thévenod F,Barbier OC

PA5-20244 was used in Immunohistochemistry to suggest that PT damage increases the uptake of the CdMT complex through 24p3R in DT (and possibly CD) and compensate for protein losses associated with AKI.

Tue Jul 06 00:00:00 UTC 2021

Increased Endocytosis of Cadmium-Metallothionein through the 24p3 Receptor in an In Vivo Model with Reduced Proximal Tubular Activity.

International journal of molecular sciences

Zavala-Guevara IP,Ortega-Romero MS,Narváez-Morales J,Jacobo-Estrada TL,Lee WK,Arreola-Mendoza L,Thévenod F,Barbier OC

Tue Jul 06 00:00:00 UTC 2021

Exposure to Iron Oxide Nanoparticles Coated with Phospholipid-Based Polymeric Micelles Induces Renal Transitory Biochemical and Histopathological Changes in Mice.

Materials (Basel, Switzerland)

Balas M,Popescu Din IM,Hermenean A,Cinteza LO,Dinischiotu A

PA5-20244 was used in Immunohistochemistry (Paraffin) to study the renal toxicity induced by the intravenously injected iron oxide nanoparticles (IONPs) encapsulated in phospholipid-based polymeric micelles.

Mon May 17 00:00:00 UTC 2021

Exposure to Iron Oxide Nanoparticles Coated with Phospholipid-Based Polymeric Micelles Induces Renal Transitory Biochemical and Histopathological Changes in Mice.

Materials (Basel, Switzerland)

Balas M,Popescu Din IM,Hermenean A,Cinteza LO,Dinischiotu A

Mon May 17 00:00:00 UTC 2021

Exposure to Iron Oxide Nanoparticles Coated with Phospholipid-Based Polymeric Micelles Induces Renal Transitory Biochemical and Histopathological Changes in Mice.

Materials (Basel, Switzerland)

Balas M,Popescu Din IM,Hermenean A,Cinteza LO,Dinischiotu A

Mon May 17 00:00:00 UTC 2021

The guanylate cyclase C agonist linaclotide ameliorates the gut-cardio-renal axis in an adenine-induced mouse model of chronic kidney disease.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Nanto-Hara F,Kanemitsu Y,Fukuda S,Kikuchi K,Asaji K,Saigusa D,Iwasaki T,Ho HJ,Mishima E,Suzuki T,Suzuki C,Tsukimi T,Matsuhashi T,Oikawa Y,Akiyama Y,Kure S,Owada Y,Tomioka Y,Soga T,Ito S,Abe T

PA5-20244 was used in Immunohistochemistry (Paraffin) to report that the laxative linaclotide decreased the circulating levels of TMAO, which in turn ameliorated renal and cardiac fibrosis, in an adenine-induced CKD model with modification of the gut microbiota.

Sat Feb 01 00:00:00 UTC 2020

Hydrogen sulfide inhibits Ca²⁺-induced mitochondrial permeability transition pore opening in type-1 diabetes.

American journal of physiology. Endocrinology and metabolism

Papu John AS,Kundu S,Pushpakumar S,Amin M,Tyagi SC,Sen U

PA5-20244 was used in Western Blotting to show that NMDA-R1-mediated Ca2+ influx in diabetes induces MPTP opening via CypD activation leading to increased oxidative stress and renal injury, and H2S protects diabetic kidney from injury by blocking mitochondrial Ca2+ permeability through NMDA-R1 pathway.

Thu Aug 01 00:00:00 UTC 2019

Prognostic value of TIM-1 expression in human non-small-cell lung cancer.

Journal of translational medicine

Zheng X,Xu K,Chen L,Zhou Y,Jiang J

PA5-20244 was used in Immunohistochemistry to determine the role of TIM-1 in the regulation of biological functions of human lung cancer cell lines.

Tue May 28 00:00:00 UTC 2019

Prognostic value of TIM-1 expression in human non-small-cell lung cancer.

Journal of translational medicine

Zheng X,Xu K,Chen L,Zhou Y,Jiang J

PA5-20244 was used in Immunohistochemistry to determine the role of TIM-1 in the regulation of biological functions of human lung cancer cell lines.

Tue May 28 00:00:00 UTC 2019

生物信息学

蛋白别名: CD365; HAVcr-1; Hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM-1; T cell immunoglobin domain and mucin domain protein 1; T-cell immunoglobulin and mucin domain-containing protein 1; T-cell immunoglobulin mucin family member 1; T-cell immunoglobulin mucin receptor 1; T-cell membrane protein 1; TIM; TIMD-1

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基因别名: CD365; HAVCR; HAVCR-1; HAVCR1; KIM-1; KIM1; TIM; TIM-1; TIM1; TIMD-1; TIMD1

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UniProt ID: (Human) Q96D42

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Entrez Gene ID: (Human) 26762

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功能

virus receptor activity

参与通路

viral entry into host cell