Invitrogen

TLR4/MD-2 Complex Monoclonal Antibody (MTS510), Functional Grade, eBioscience™

TLR4/MD-2 Complex Antibody in Flow Cytometry (Flow)

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TLR4/MD-2 Complex Antibody (16-9924-82) in Flow

Staining of thioglycolate induced PECs with Anti-Mouse TLR4/MD-2 Complex PE Appropriate isotype controls were used (open histogram). Cells in the large scatter population were used for analysis.

TLR4/MD-2 Complex Antibody in Immunocytochemistry (ICC/IF)

TLR4/MD-2 Complex Antibody in Immunohistochemistry (IHC)

TLR4/MD-2 Complex Antibody in Western Blot (WB)

TLR4/MD-2 Complex Antibody in Neutralization (Neu)

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产品信息

16-9924-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

1 µg/test

查看6篇已发表文章 6篇已发表文章

酶联免疫吸附实验 (ELISA)

查看1篇已发表文章 1篇已发表文章

中和实验 (Neu)

Assay-Dependent

查看5篇已发表文章 5篇已发表文章

功能检测 (Functional)

Assay-Dependent

产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

MTS510

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

AB_469285

产品详细信息

Description: The MTS510 monoclonal antibody reacts with the mouse Toll-like receptor 4 (TLR4)/MD-2 complex. At least ten members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. In the mouse, TLR4 is expressed by thioglycolate-elicited peritoneal macrophages. Incubation of peritoneal macrophages with LPS results in down regulation of surface TLR4/MD-2. The TLR4 gene is defective in C3H/HeJ and C57BL/10ScCr mice, both of which have been well characterized as hyporesponders to LPS.

The MTS510 monoclonal antibody co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa), and preferentially reacts with TLR4 that is associated with MD-2. In comparison, binding of the UT41 monoclonal antibody occurs with and without formation of the TLR4/MD-2 complex. Please contact eBioscience Technical Support for further information.

Applications Reported: MTS510 has been reported for use in flow cytometric analysis and inhibition of LPS-induced cytokine production.

Applications Tested: The MTS510 antibody has been tested by flow cytometric analysis of mouse peritoneal exudates cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Hydroxychloroquine attenuates neuroinflammation following traumatic brain injury by regulating the TLR4/NF-κB signaling pathway.

Journal of neuroinflammation

Hu J,Wang X,Chen X,Fang Y,Chen K,Peng W,Wang Z,Guo K,Tan X,Liang F,Lin L,Xiong Y

Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 16-9924-82) in Western Blot

Mon Mar 28 00:00:00 UTC 2022

Lycopene Inhibits Toll-Like Receptor 4-Mediated Expression of Inflammatory Cytokines in House Dust Mite-Stimulated Respiratory Epithelial Cells.

Molecules (Basel, Switzerland)

Choi J,Lim JW,Kim H

Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 16-9924-82) in Immunocytochemistry

Mon May 24 00:00:00 UTC 2021

Inflammatory signaling in dengue-infected platelets requires translation and secretion of nonstructural protein 1.

Blood advances

Quirino-Teixeira AC,Rozini SV,Barbosa-Lima G,Coelho DR,Carneiro PH,Mohana-Borges R,Bozza PT,Hottz ED

16-9924 was used in Neutralization experiments to reveal distinct, new mechanisms for platelet activation in dengue, involving DENV genome translation and NS1-induced platelet activation via platelet TLR4.

Tue May 12 00:00:00 UTC 2020

Inflammatory signaling in dengue-infected platelets requires translation and secretion of nonstructural protein 1.

Blood advances

Quirino-Teixeira AC,Rozini SV,Barbosa-Lima G,Coelho DR,Carneiro PH,Mohana-Borges R,Bozza PT,Hottz ED

Tue May 12 00:00:00 UTC 2020

Chanzyme TRPM7 Mediates the Ca²⁺ Influx Essential for Lipopolysaccharide-Induced Toll-Like Receptor 4 Endocytosis and Macrophage Activation.

Immunity

Schappe MS,Szteyn K,Stremska ME,Mendu SK,Downs TK,Seegren PV,Mahoney MA,Dixit S,Krupa JK,Stipes EJ,Rogers JS,Adamson SE,Leitinger N,Desai BN

Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 16-9924-82) in Flow Cytometry

Tue Jan 16 00:00:00 UTC 2018

High mobility group box 1 induces calcification of aortic valve interstitial cells via toll-like receptor 4.

Molecular medicine reports

Shen W,Zhou J,Wang C,Xu G,Wu Y,Hu Z

16-9924 was used in Neutralization experiments to investigate the mechanisms underlying chronic inflammation and the calcification of aortic valve interstitial cells in calcific aortic valve disease.

Mon May 01 00:00:00 UTC 2017

Green Tea Polyphenol Epigallocatechin-3-gallate Suppresses Toll-like Receptor 4 Expression via Up-regulation of E3 Ubiquitin-protein Ligase RNF216.

The Journal of biological chemistry

Kumazoe M,Nakamura Y,Yamashita M,Suzuki T,Takamatsu K,Huang Y,Bae J,Yamashita S,Murata M,Yamada S,Shinoda Y,Yamaguchi W,Toyoda Y,Tachibana H

Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 16-9924-82) in Flow Cytometry

Fri Mar 10 00:00:00 UTC 2017

Identification of a novel mechanism of blood-brain communication during peripheral inflammation via choroid plexus-derived extracellular vesicles.

EMBO molecular medicine

Balusu S,Van Wonterghem E,De Rycke R,Raemdonck K,Stremersch S,Gevaert K,Brkic M,Demeestere D,Vanhooren V,Hendrix A,Libert C,Vandenbroucke RE

Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 16-9924-82) in Immunofluorescence

Sat Oct 01 00:00:00 UTC 2016

Chronic exposure to low dose bacterial lipopolysaccharide inhibits leptin signaling in vagal afferent neurons.

Physiology & behavior

de La Serre CB,de Lartigue G,Raybould HE

Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 16-9924-82) in Western Blot

Sun Feb 01 00:00:00 UTC 2015

Chronic exposure to low dose bacterial lipopolysaccharide inhibits leptin signaling in vagal afferent neurons.

Physiology & behavior

de La Serre CB,de Lartigue G,Raybould HE

Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 16-9924-82) in Western Blot

Sun Feb 01 00:00:00 UTC 2015

Toll-like receptor 4 mediates inflammatory cytokine secretion in smooth muscle cells induced by oxidized low-density lipoprotein.

PloS one

Yang K,Zhang XJ,Cao LJ,Liu XH,Liu ZH,Wang XQ,Chen QJ,Lu L,Shen WF,Liu Y

Tue Jan 20 00:00:00 UTC 2015

Toll-like receptor 4 mediates inflammatory cytokine secretion in smooth muscle cells induced by oxidized low-density lipoprotein.

PloS one

Yang K,Zhang XJ,Cao LJ,Liu XH,Liu ZH,Wang XQ,Chen QJ,Lu L,Shen WF,Liu Y

Tue Jan 20 00:00:00 UTC 2015

Toll-like receptor 4 mediates inflammatory cytokine secretion in smooth muscle cells induced by oxidized low-density lipoprotein.

PloS one

Yang K,Zhang XJ,Cao LJ,Liu XH,Liu ZH,Wang XQ,Chen QJ,Lu L,Shen WF,Liu Y

Tue Jan 20 00:00:00 UTC 2015

Intratumoral delivery of low doses of anti-CD40 mAb combined with monophosphoryl lipid a induces local and systemic antitumor effects in immunocompetent and T cell-deficient mice.

Journal of immunotherapy (Hagerstown, Md. : 1997)

Van De Voort TJ,Felder MA,Yang RK,Sondel PM,Rakhmilevich AL

16-9924 was used in Flow cytometry/Cell sorting to assess the immunomodulatory and antitumour synergy between an agonistic anti-CD40 monoclonal antibody and monophosphoryl lipid A in mice.

Tue Jan 01 00:00:00 UTC 2013

Inefficient TLR4/MD-2 heterotetramerization by monophosphoryl lipid A.

PloS one

Casella CR,Mitchell TC

Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 16-9924-82) in Flow Cytometry

Mon Nov 11 00:00:00 UTC 2013

An extract of Rhodobacter sphaeroides reduces cisplatin-induced nephrotoxicity in mice.

Toxins

Chang WW,Liu JJ,Liu CF,Liu WS,Lim YP,Cheng YJ,Lee CH

16-9924 was used in an ELISA assay to evaluate the effect of Lycogen™, a potent anti-inflammatory agent, in mice with cisplatin-induced renal injury.

Fri Nov 29 00:00:00 UTC 2013

Clearance of fetuin-A--containing calciprotein particles is mediated by scavenger receptor-A.

Circulation research

Herrmann M,Schäfer C,Heiss A,Gräber S,Kinkeldey A,Büscher A,Schmitt MM,Bornemann J,Nimmerjahn F,Herrmann M,Helming L,Gordon S,Jahnen-Dechent W

16-9924 was used in Blocking experiments to identify the tissues, cells, and receptors involved in the clearance of calciprotein particles.

Fri Aug 17 00:00:00 UTC 2012

Defective antiviral responses of induced pluripotent stem cells to baculoviral vector transduction.

Journal of virology

Chen GY,Hwang SM,Su HJ,Kuo CY,Luo WY,Lo KW,Huang CC,Chen CL,Yu SH,Hu YC

Published figure using TLR4/MD-2 Complex monoclonal antibody (Product # 16-9924-82) in Flow Cytometry

Wed Aug 01 00:00:00 UTC 2012

Persistence versus escape: Aspergillus terreus and Aspergillus fumigatus employ different strategies during interactions with macrophages.

PloS one

Slesiona S,Gressler M,Mihlan M,Zaehle C,Schaller M,Barz D,Hube B,Jacobsen ID,Brock M

16-9924 was used in Blocking experiments to show that A. terreus and A. fumigatus have evolved significantly different host immune evasion strategies.

Mon Sep 24 00:00:00 UTC 2012

Proinflammatory clearance of apoptotic neutrophils induces an IL-12(low)IL-10(high) regulatory phenotype in macrophages.

Journal of immunology (Baltimore, Md. : 1950)

Filardy AA,Pires DR,Nunes MP,Takiya CM,Freire-de-Lima CG,Ribeiro-Gomes FL,DosReis GA

16-9924 was used in Neutralization experiments to suggest that macrophage differentiation to a regulatory phenotype plays a role in resolution of inflammation.

Sun Aug 15 00:00:00 UTC 2010

TLRs regulate the gatekeeping functions of the intestinal follicle-associated epithelium.

Journal of immunology (Baltimore, Md. : 1950)

Chabot S,Wagner JS,Farrant S,Neutra MR

Sat Apr 01 00:00:00 UTC 2006

生物信息学

蛋白别名: ARMD10; CD284; ESOP-1; hToll; lipopolysaccharide response; Ly-96; Lymphocyte antigen 96; myeloid differentiation factor-2; myeloid differentiation protein-2; Protein MD-2; TLR-4; TOLL; Toll-like receptor 4

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基因别名: ESOP-1; Esop1; Lps; Ly87; Ly96; MD-2; MD2; Ran/M1; Rasl2-8; Tlr4

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UniProt ID: (Mouse) Q9JHF9, (Mouse) Q9QUK6

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Entrez Gene ID: (Mouse) 17087, (Mouse) 21898

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功能

lipopolysaccharide receptor activity protein binding lipopolysaccharide binding receptor activity transmembrane signaling receptor activity phosphatidylinositol 3-kinase binding scaffold/adaptor protein

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