Invitrogen

TRAP1 Polyclonal Antibody

TRAP1 Antibody in Immunocytochemistry (ICC/IF)

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TRAP1 Antibody (PA5-30358) in ICC/IF

Immunofluorescence analysis of TRAP1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TRAP1 Polyclonal Antibody (Product # PA5-30358) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2703... View More

TRAP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TRAP1 Antibody — Antibody specificity was demonstrated by siRNA mediated KD of target protein. HeLa cells were transfected with TRAP1 siRNA and reduction of specific signal was observed in Immunofluorescence application using TRAP1 Rabbit Polyclonal Antibody (Product # PA5-30358). {KD}

产品信息

PA5-30358

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 22 and 236 of Human TRAP1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.9 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2547832

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2.

Predicted reactivity: Mouse (91%), Rat (90%), Zebrafish (81%), Pig (91%), Chicken (88%), Chimpanzee (100%), Bovine (89%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

The 90 kDa heat shock protein (hsp90) family of molecular chaperones is a highly conserved family of proteins that play an important physiological role. Hsp90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of hsp90 is the tumor necrosis factor receptor-associated protein (TRAP1). Like hsp90, TRAP1 is found to be associated with numerous proteins involved in diverse actions. Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting presequence strongly implicates TRAP1 as a mitochondrial matrix protein.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Heat shock protein 75 kDa, mitochondrial; Heat shock protein family C member 5; HSP 75; testicular tissue protein Li 209; TNF receptor-associated protein 1; TNFR-associated protein 1; TRAP-1; Tumor necrosis factor type 1 receptor-associated protein

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基因别名: 2410002K23Rik; HSP 75; HSP75; HSP90L; HSPC5; TRAP-1; TRAP1

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UniProt ID: (Human) Q12931, (Mouse) Q9CQN1

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Entrez Gene ID: (Human) 10131, (Mouse) 68015

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功能

tumor necrosis factor receptor binding protein binding ATP binding protein kinase binding poly(A) RNA binding unfolded protein binding nucleotide binding Hsp90 family chaperone

参与通路

response to stress translational attenuation chaperone-mediated protein folding negative regulation of cellular respiration negative regulation of reactive oxygen species biosynthetic process negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide protein folding negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway