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Themis Antibody (MA5-24337) in WB
Western blot analysis of Themis in mouse thymus tissue. Samples were incubated in Themis monoclonal antibody (Product # MA5-24337) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Rat IgG secondary antibody. A specific band was detected for Themis at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions.
产品信息
MA5-24337
产品规格
种属反应
Mouse
宿主/亚型
Rat
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
719945
抗原
E. coli-derived recombinant mouse Themis Lys122-Lys237
偶联物
形式
Lyophilized
浓度
0.5 mg/mL
纯化类型
Protein A/G
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2606930
产品详细信息
In Western blots, 100% cross-reactivity with recombinant human (rh) Themis (aa 2-282) is observed under non-reducing conditions, and approximately 10% cross-reactivity with rhThemis (aa 2-282) is observed under reducing conditions.
Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
靶标信息
Themis is a cytoplasmic protein that is essential for positive selection and the development of single-positive thymocytes. Highest levels of expression are found in CD4+CD8+ double-positive thymocytes, with lower expression found in mature T cells. In addition to Grb1, Themis has been found to associate with PLC gamma 1 and LAT and is quickly phosphorylated by SLP-76 following TCR signaling. There are 5 isoforms of mouse Themis that are produced as a result of alternative splicing.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: bA325O24.3; bA325O24.4; FLJ40584; Gasp; Grb2-associating protein; MGC163388; Protein THEMIS; Protein thylex; Thymocyte-expressed molecule involved in selection
基因别名: E430004N04Rik; Gasp; Spot; Themis; thylex; Tsepa
UniProt ID: (Mouse) Q8BGW0
Entrez Gene ID: (Mouse) 210757
