Invitrogen

Thymidylate Synthase Polyclonal Antibody

Thymidylate Synthase Antibody in Immunocytochemistry (ICC/IF)

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Thymidylate Synthase Antibody (PA5-27867) in ICC/IF

Thymidylate Synthase Polyclonal Antibody detects Thymidylate synthase protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Thymidylate synthase stained by Thymidylate Synthase Polyclonal Antibody (Product # PA5-27867) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin Polyclonal Antibody [GT114] (... View More

Thymidylate Synthase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Thymidylate Synthase Antibody in Western Blot (WB)

Thymidylate Synthase Antibody in Immunoprecipitation (IP)

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产品信息

PA5-27867

应用

建议稀释比

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免疫印迹 (WB)

1:500-1:3,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

免疫沉淀 (IP)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 35 and 313 of Human Thymidylate synthetase

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2545343

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, Jurkat, Raji, THP-1, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, PC-12, Rat2.

Predicted reactivity: Mouse (94%), Rat (94%), Zebrafish (86%), Xenopus laevis (85%), Dog (94%), Pig (95%), Rhesus Monkey (98%), Chimpanzee (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Thymidylate Synthase (TS) converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。