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USP19 Antibody (PA5-101943) in ICC/IF
Immunofluorescent analysis of USP19 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and USP19 polyclonal antibody (Product # PA5-101943) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-101943
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human USP19(Accession O94966), corresponding to amino acid residues A396-T446.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2851375
产品详细信息
Antibody detects endogenous levels of total USP19.
靶标信息
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs), 1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Deubiquitinating enzyme 19; Ubiquitin carboxyl-terminal hydrolase 19; ubiquitin specific protease 19; Ubiquitin thioesterase 19; ubiquitin thiolesterase 19; ubiquitin-specific processing protease 19; Ubiquitin-specific-processing protease 19; Zinc finger MYND domain-containing protein 9
基因别名: 8430421I07Rik; AI047774; KIAA0891; USP19; ZMYND9
UniProt ID: (Human) O94966, (Rat) Q6J1Y9, (Mouse) Q3TUE6
Entrez Gene ID: (Human) 10869, (Rat) 361190, (Mouse) 71472
thiol-dependent ubiquitin-specific protease activity
protein binding
ubiquitin protein ligase binding
metal ion binding
Hsp90 protein binding
Lys48-specific deubiquitinase activity
cysteine-type endopeptidase activity
peptidase activity
cysteine-type peptidase activity
hydrolase activity
thiol-dependent ubiquitinyl hydrolase activity
cysteine protease
protein deubiquitination
ER-associated ubiquitin-dependent protein catabolic process
regulation of protein stability
response to endoplasmic reticulum stress
negative regulation of skeletal muscle tissue development
protein stabilization
protein K48-linked deubiquitination
positive regulation of cell cycle process
regulation of cellular response to hypoxia
negative regulation of proteasomal protein catabolic process
regulation of ERAD pathway
proteolysis
regulation of proteasomal protein catabolic process
ubiquitin-dependent protein catabolic process
