Invitrogen

Vimentin Monoclonal Antibody (V9), Alexa Fluor™ 420

Vimentin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Vimentin Antibody (758-9897-82) in IHC (P)

Immunohistochemical analysis of Vimentin was performed on formalin-fixed paraffin-embedded human tonsil tissue. To expose the target protein, HIER was performed on de-paraffinized sections using BOND Epitope Retrieval Solution 2 (pH 9) for 10 minutes, followed by a 5-min cool down and a 5-min wash with ddH2O. Tissues were permeabilized with 0.1% Triton X-100 in 1X PBS for 30 mins and blocked with 3% BSA/5% ... View More

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产品信息

758-9897-82

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

2.5 µg/mL

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

偶联物

Alexa Fluor™ 420 Alexa Fluor™ 420 Alexa Fluor™ 420

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.5% BSA, 10% proprietary stabilizer

内含物

0.05% sodium azide

保存条件

4° C, store in dark

运输条件

Wet ice

产品详细信息

Description: The V9 monoclonal antibody recognizes human Vimentin, a 57 kDa protein that functions as a structural component of intermediate filaments. Vimentin is expressed in cells derived from the mesenchyme but also in specific populations such as radial glia and immature glial cells, pancreatic precursor cells. It is proposed to be a marker of cardiac differentiation. In neural cells, vimentin expression is gradually replaced by neurofilaments. Reports have also shown surface expression of vimentin on activated macrophages, platelets, as well as apoptotic T cells and neutrophils.

This antibody also recognizes canine (dog), rat and chicken vimentin but does not recognize mouse vimentin.

Applications Reported: This V9 antibody has been reported for use in western blotting, immunocytochemistry, and immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded tissue sections (IHC-P).

Applications Tested: This V9 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using high pH antigen retrieval and can be used at 2.5 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

靶标信息

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。