Invitrogen

ZNF276 Polyclonal Antibody

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1 篇参考文献

抗体检测数据 (1)
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ZNF276 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ZNF276 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ZNF276 Antibody (PA5-54578) in IHC (P)

Immunohistochemical analysis of ZNF276 in human tonsil using ZNF276 Polyclonal Antibody (Product # PA5-54578) shows strong nuclear positivity in cells outside the reaction centra.

ZNF276 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ZNF276 Antibody in Western Blot (WB)

ZNF276 Antibody in Western Blot (WB)

ZNF276 Antibody in Western Blot (WB)

ZNF276 Antibody in Western Blot (WB)

Product Image Gallery

ZNF276 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of ZNF276 in human tonsil using ZNF276 Polyclonal Antibody (Product # PA5-54578) shows strong nuclear positivity in cells outside the reaction centra.

ZNF276 Antibody in Western Blot (WB) — Expression and prognosis of ZNF276 in breast cancer tissues and cell lines. A mRNA expression of ZNF276 in different malignancies and normal tissues from TCGA database. BRCA breast carcinoma. CESC Cervical and endocervical carcinoma. COAD Colon adenocarcinoma. LIHC Liver hepatocellular carcinoma. LUAD Lung adenocarcinoma. STAD Stomach adenocarcinoma. UCEC Uterine Corpus Endometrial Carcinoma. B , C RT-qPCR ( B ) and western blot ( C ) analysis of ZNF276 expression in 10 breast cancer tissues and matched surrounding normal tissues. D Representative pictures of ZNF276 expression in 118 breast cancer tissues and 48 adjacent tissues from tissue chips. E , F Statistical analysis of ZNF276 protein expression in breast cancer tissues and adjacent tissues ( E ) and different molecular subtypes ( F ) from tissue chips by H-score. G ZNF276 mRNA expressions in different breast cancer cell lines were obtained from the CCLE portal. H Protein expression of ZNF276 was detected in normal breast cells and six breast cancer cells by western blot. I Correlation between ZNF276 expression and prognosis of breast cancer patients from TCGA dataset. J Kaplan-Meier curve showing the overall survival of 132 breast cancer patients separated by high and low ZNF276 expression. * p < 0.05; ** p < 0.01; *** p < 0.001.

ZNF276 Antibody in Western Blot (WB) — Fig. 7 ZNF276 activates Wnt/beta-catenin signaling through recruitment of MAGEB2 to regulate CYP1B1 transcription. A GO analysis of the potential ZNF276-interacted proteins. B KEGG analysis of the potential ZNF276-interacted proteins. C , D Both forward ( C ) and reverse ( D ) co-IP analysis affirmed the interaction between ZNF276 and MAGEB2. E ChIP-PCR analysis of ZNF276 binding ability at the CYP1B1 promoter affected by MAGEB2 overexpression. F , G The luciferase activity of CYP1B1 promoter decreased by ZNF276 silencing was further enhanced by the expression of MAGEB2 in 293T and MCF-7. H , I RT-qPCR ( H ) and western blot ( I ) analysis confirmed the effect of MAGEB2 overexpression in CYP1B1 mRNA and protein expression in MCF-7 cells. J , K The reduced transcriptional activity of CYP1B1 promoter by ZNF276 was partially reversed by MAGEB2. L , M Overexpression of MAGEB2 reversed the decreased cell proliferation, migration and invasion abilities affected by ZNF276 silencing in CCK-8 assay ( L ), transwell migration ( M , up) and transwell invasion ( M , down).

ZNF276 Antibody in Western Blot (WB) — Fig. 2 ZNF276 promotes the proliferation of breast cancer cells. A Western blot identifying the ZNF276 overexpression in MDA-MB-231 and SK-BR-3, ZNF276 knockdown in MCF-7 and ZNF276 knockout in UACC-812. B-C. CCK-8 assay measuring the effect of ZNF276 overexpression on cell proliferation in MDA-MB-231 ( B ) and SK-BR-3 ( C ) cells. D , E The effect of ZNF276 silencing ( D ) and knockout ( E ) on cell proliferation was evaluated by CCK-8 assay. F Cell proliferation ability of ZNF276 overexpressing cells was measured by clone formation assay. G Clone formation assay measuring the effect of ZNF276 silencing and knockout on cell proliferation. H ZNF276 overexpression affecting breast cancer tumor formation in the mammary fat pad of nude mice. I Measurement of tumor volume of the resected tumors from mice injected with ZNF276 overexpressing cells and control cells. J Statistical analysis of tumor weight of the resected tumors. K Immunohistochemical staining of Ki67 and ZNF276 in the resected tumors. Bar = 40 um. * p < 0.05; ** p < 0.01; *** p < 0.001.

ZNF276 Antibody in Western Blot (WB) — Fig. 5 ZNF276 activates the Wnt/beta-catenin signaling by regulation of CYP1B1. A , C Western blotting ( A ) and RT-qPCR ( C ) analysis revealed that silencing of CYP1B1 inhibited the expression of c-Myc and Cyclin D1 enhanced by ZNF276 overexpression in MDA-MB-231 cells. B , D Effects of ZNF276 knockdown and CYP1B1 overexpression on c-Myc and Cyclin D1 expression were analyzed in UACC-812 cells by western blotting ( B ) and RT-qPCR ( D ). E , F ZNF276 lacking C 2 H 2 domain inhibited the TOP Flash activity enhanced by the intact ZNF276 with wnt3a activation in MDA-MB-231 ( E ) and UACC-812 ( F ) cells. G Endogenous beta-catenin expression and distribution was observed by immunofluorescence in MDA-MB-231 cells transfected with intact ZNF276 or ZNF276 lacking C 2 H 2 domain plasmid. Nuc nuclear. Bar = 20 um. * p < 0.05; ** p < 0.01; *** p < 0.001.

产品信息

PA5-54578

应用

建议稀释比

已发表文章

免疫印迹 (WB)

-

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免疫组化（石蜡） (IHC (P))

1:50-1:200

-

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human ZNF276. Recombinant protein control fragment (Product #RP-93590).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2650037

产品详细信息

Immunogen sequence: PWDKETAPRL PQHRGWNPGD APQTSQGRGT GTPVGAETKT LPSTDVAQPP SDSDAVGPRS GFPPQPSLPL CRAPGQLGEK QLPSSTSDDR

Highest antigen sequence identity to the following orthologs: Mouse - 55%, Rat - 57%.

靶标信息

May be involved in transcriptional regulation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

ZNF276 promotes the malignant phenotype of breast carcinoma by activating the CYP1B1-mediated Wnt/β-catenin pathway.

Cell death & disease

Lei T,Zhang W,He Y,Wei S,Song X,Zhu Y,Luo G,Kuang Z,Li G,Zhou Q,Sun Z,Xiao B,Li L

PA5-54578 was used in Immunoprecipitation to highlight the oncogenic role of ZNF276 on breast cancer cell proliferation and metastasis.

Sat Sep 10 00:00:00 UTC 2022

ZNF276 promotes the malignant phenotype of breast carcinoma by activating the CYP1B1-mediated Wnt/β-catenin pathway.

Cell death & disease

Lei T,Zhang W,He Y,Wei S,Song X,Zhu Y,Luo G,Kuang Z,Li G,Zhou Q,Sun Z,Xiao B,Li L

PA5-54578 was used in Immunoprecipitation to highlight the oncogenic role of ZNF276 on breast cancer cell proliferation and metastasis.

Sat Sep 10 00:00:00 UTC 2022

生物信息学

蛋白别名: centromere protein Z; Zfp-276; Zinc finger protein 276; zinc finger protein 276 homolog; Zinc finger protein 477; zinc finger, AD-type

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基因别名: CENP-Z; CENPZ; ZADT; ZFP276; ZNF276; ZNF477

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UniProt ID: (Human) Q8N554

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Entrez Gene ID: (Human) 92822

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DNA binding protein binding zinc ion binding C2H2 zinc finger transcription factor

参与通路

transcription, DNA-templated regulation of transcription, DNA-templated