Invitrogen

ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor™ 647

ZO-1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 4

ZO-1 Antibody (MA3-39100-A647) in ICC/IF

Immunofluorescent analysis of ZO-1 (red) in Caco-2 cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a ZO-1 Monoclonal Antibody, AlexaFluor 647 conjugate (Product # MA3-39100-A647) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Imag... View More

Product Image Gallery

产品信息

MA3-39100-A647

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

查看1篇已发表文章 1篇已发表文章

免疫组织化学（多聚甲醛固定） (IHC (PFA))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1-10 µg/mL

查看5篇已发表文章 5篇已发表文章

流式细胞分析 (Flow)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Dog, Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

ZO1-1A12

抗原

Human recombinant ZO-1 fusion protein encompassing amino acids 334-634

查看免疫原

偶联物

Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647

激发/发射光谱

650/671 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2663167

靶标信息

ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. ZO-1 may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Prime editing-mediated correction of the CFTR W1282X mutation in iPSCs and derived airway epithelial cells.

PloS one

Li C,Liu Z,Anderson J,Liu Z,Tang L,Li Y,Peng N,Chen J,Liu X,Fu L,Townes TM,Rowe SM,Bedwell DM,Guimbellot J,Zhao R

MA3-39100-A647 was used in Immunocytochemistry to demonstrate that prime editing may potentially be applied to correct CFTR mutations such as W1282X. Copyright:.

Fri Dec 01 00:00:00 UTC 2023

Signal transduction and targeted therapy

Shi X,Wang Z,Ren W,Chen L,Xu C,Li M,Fan S,Xu Y,Chen M,Zheng F,Zhang W,Zhou X,Zhang Y,Qiu S,Wu L,Zhou P,Lv X,Cui T,Qiao Y,Zhao H,Guo W,Chen W,Li S,Zhong W,Lin J,Yang S

MA3-39100-A647 was used in Immunohistochemistry-immunofluorescence to develop an LRP1-binding peptide, IETP2, and covalently conjugated a series of model small-molecule compounds to it, including potential drugs and imaging agents.

Fri Jun 10 00:00:00 UTC 2022

Nephrin expression in human epidermal keratinocytes and its implication in poor wound closure.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Kim JY,Lee EJ,Seo J,Lee Y,Ahn Y,Park S,Bae YJ,Lee J,Lim BJ,Kim D,Cho JW,Oh SH

MA3-39100-A647 was used in Immunohistochemistry-immunofluorescence to suggest that nephrin may have a variety of physiological roles in human skin.

Fri Jul 01 00:00:00 UTC 2022

Culture and Imaging of Human Nasal Epithelial Organoids.

Journal of visualized experiments : JoVE

Liu Z,Anderson JD,Natt J,Guimbellot JS

MA3-39100-A647 was used in Immunocytochemistry to understand baseline Cystic Fibrosis Transmembrane conductance Regulator (CFTR) activity and restoration from small molecule compounds.

Fri Dec 17 00:00:00 UTC 2021

Human Nasal Epithelial Organoids for Therapeutic Development in Cystic Fibrosis.

Genes

Liu Z,Anderson JD,Deng L,Mackay S,Bailey J,Kersh L,Rowe SM,Guimbellot JS

MA3-39100-A647 was used in Immunocytochemistry to assess correction of the mutant gene in CF or other diseases, recapitulating the phenotypic and genotypic diversity of the population.

Fri May 29 00:00:00 UTC 2020

Human Nasal Epithelial Organoids for Therapeutic Development in Cystic Fibrosis.

Genes

Liu Z,Anderson JD,Deng L,Mackay S,Bailey J,Kersh L,Rowe SM,Guimbellot JS

MA3-39100-A647 was used in Immunocytochemistry to assess correction of the mutant gene in CF or other diseases, recapitulating the phenotypic and genotypic diversity of the population.

Fri May 29 00:00:00 UTC 2020

Mitochondrial phosphatase PGAM5 modulates cellular senescence by regulating mitochondrial dynamics.

Nature communications

Yu B,Ma J,Li J,Wang D,Wang Z,Wang S

MA3-39100-A647 was used in Immunohistochemistry (PFA fixed) to demonstrate that deletion of mitochondrial phosphatase PGAM5 leads to accelerated retinal pigment epithelial (RPE) senescence in vitro and in vivo.

Thu May 21 00:00:00 UTC 2020

Mitochondrial phosphatase PGAM5 modulates cellular senescence by regulating mitochondrial dynamics.

Nature communications

Yu B,Ma J,Li J,Wang D,Wang Z,Wang S

Thu May 21 00:00:00 UTC 2020

Mitochondrial phosphatase PGAM5 modulates cellular senescence by regulating mitochondrial dynamics.

Nature communications

Yu B,Ma J,Li J,Wang D,Wang Z,Wang S

Thu May 21 00:00:00 UTC 2020

Comparative Analysis of Cell-Cell Contact Abundance in Ovarian Carcinoma Cells Cultured in Two- and Three-Dimensional In Vitro Models.

Biology

Kutova OM,Sencha LM,Pospelov AD,Dobrynina OE,Brilkina AA,Cherkasova EI,Balalaeva IV

MA3-39100-A647 was used in Flow cytometry/Cell sorting to compare the abundance of contact proteins in SKOV-3 and SKOV-3.ip human ovarian adenocarcinoma cell lines, cultivated in monolayers, tumor spheroids and collagen hydrogels.

Fri Dec 04 00:00:00 UTC 2020

Comparative Analysis of Cell-Cell Contact Abundance in Ovarian Carcinoma Cells Cultured in Two- and Three-Dimensional In Vitro Models.

Biology

Kutova OM,Sencha LM,Pospelov AD,Dobrynina OE,Brilkina AA,Cherkasova EI,Balalaeva IV

Fri Dec 04 00:00:00 UTC 2020

Inhibition of MicroRNA-155 Supports Endothelial Tight Junction Integrity Following Oxygen-Glucose Deprivation.

Journal of the American Heart Association

Pena-Philippides JC,Gardiner AS,Caballero-Garrido E,Pan R,Zhu Y,Roitbak T

MA3-39100-A647 was used in Immunocytochemistry-immunoflourescence to investigate the influence of microRNA-155 on the barrier characteristics of human primary brain microvascular endothelial cells.

Tue Jun 26 00:00:00 UTC 2018

生物信息学

蛋白别名: DKFZp686M05161; MGC133289; OTTHUMP00000174520; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1

查看更多收起

基因别名: TJP1; ZO-1; ZO1

查看更多收起

UniProt ID: (Dog) O97758, (Human) Q07157

查看更多收起

Entrez Gene ID: (Dog) 403752, (Human) 7082

查看更多收起

功能

protein binding calmodulin binding protein domain specific binding cadherin binding involved in cell-cell adhesion tight junction

参与通路

bicellular tight junction assembly cellular response to calcium ion blastocyst formation cell-cell junction assembly sensory perception of sound hippo signaling establishment of endothelial intestinal barrier cell-cell adhesion cell-cell signaling involved in cell-cell junction organization