Invitrogen

alpha Actinin 2 Recombinant Rabbit Monoclonal Antibody (7H1L69)

alpha Actinin 2 Antibody in Immunocytochemistry (ICC/IF)

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alpha Actinin 2 Antibody (701914) in ICC/IF

For immunofluorescence analysis, HSkM cells were fixed and permeabilized for detection of endogenous sarcomeric alpha actinin using Anti-sarcomeric alpha actinin Recombinant Rabbit Monoclonal Antibody (Product # 701914, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2,000). Panel a) shows representative cells that... View More

alpha Actinin 2 Antibody in Immunohistochemistry (Frozen) (IHC (F))

alpha Actinin 2 Antibody in Western Blot (WB)

alpha Actinin 2 Antibody in Western Blot, Immunoprecipitation (WB, IP)

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产品信息

701914

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

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免疫组化（冰冻） (IHC (F))

2 µg/mL

免疫细胞化学 (ICC/IF)

1:100

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免疫沉淀 (IP)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

7H1L69

抗原

Peptides corresponding to human Sarcomeric alpha actinin/alpha actinin 2 [1) aa834-aa852, 2) aa5-aa24]

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2688290

产品详细信息

This antibody is predicted to react with Monkey, Pig, Rat, Mouse

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it i involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Induction of filopodia formation by α-Actinin-2 via RelA with a feedforward activation loop promoting overt bone marrow metastasis of gastric cancer.

Journal of translational medicine

Wang C,Xie B,Yin S,Cao J,Huang J,Jin L,Du G,Zhai X,Zhang R,Li S,Cao T,Yu H,Fan X,Yang Z,Peng J,Xiao J,Lian L

701914 was used in Immunocytochemistry-immunofluorescence to propose ACTN2 as a druggable molecular vulnerability that may provide potential therapeutic benefit against BMM of GC.

Mon Jun 19 00:00:00 UTC 2023

Induction of filopodia formation by α-Actinin-2 via RelA with a feedforward activation loop promoting overt bone marrow metastasis of gastric cancer.

Journal of translational medicine

Wang C,Xie B,Yin S,Cao J,Huang J,Jin L,Du G,Zhai X,Zhang R,Li S,Cao T,Yu H,Fan X,Yang Z,Peng J,Xiao J,Lian L

701914 was used in Immunocytochemistry-immunofluorescence to propose ACTN2 as a druggable molecular vulnerability that may provide potential therapeutic benefit against BMM of GC.

Mon Jun 19 00:00:00 UTC 2023

Induction of filopodia formation by α-Actinin-2 via RelA with a feedforward activation loop promoting overt bone marrow metastasis of gastric cancer.

Journal of translational medicine

Wang C,Xie B,Yin S,Cao J,Huang J,Jin L,Du G,Zhai X,Zhang R,Li S,Cao T,Yu H,Fan X,Yang Z,Peng J,Xiao J,Lian L

701914 was used in Immunocytochemistry-immunofluorescence to propose ACTN2 as a druggable molecular vulnerability that may provide potential therapeutic benefit against BMM of GC.

Mon Jun 19 00:00:00 UTC 2023

Hyperglycaemia-induced impairment of the autorhythmicity and gap junction activity of mouse embryonic stem cell-derived cardiomyocyte-like cells.

Histochemistry and cell biology

Menzele A,Aboalgasm H,Ballo R,Gwanyanya A

701914 was used in Immunocytochemistry to investigate the effect of hyperglycaemia on the pulsatile activity of stem cell-derived cardiomyocytes.

Sat Apr 01 00:00:00 UTC 2023

A multi-organ chip with matured tissue niches linked by vascular flow.

Nature biomedical engineering

Ronaldson-Bouchard K,Teles D,Yeager K,Tavakol DN,Zhao Y,Chramiec A,Tagore S,Summers M,Stylianos S,Tamargo M,Lee BM,Halligan SP,Abaci EH,Guo Z,Jacków J,Pappalardo A,Shih J,Soni RK,Sonar S,German C,Christiano AM,Califano A,Hirschi KK,Chen CS,Przekwas A,Vunjak-Novakovic G

701914 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot, Immunohistochemistry (Paraffin) to report the development and applicability of a tissue-chip system in which matured human heart, liver, bone and skin tissue niches are linked by recirculating vascular flow to allow for the recapitulation of interdependent organ functions.

Fri Apr 01 00:00:00 UTC 2022

Deep learning detects cardiotoxicity in a high-content screen with induced pluripotent stem cell-derived cardiomyocytes.

eLife

Grafton F,Ho J,Ranjbarvaziri S,Farshidfar F,Budan A,Steltzer S,Maddah M,Loewke KE,Green K,Patel S,Hoey T,Mandegar MA

701914 was used in Immunocytochemistry to screen a library of 1280 bioactive compounds and identified those with potential cardiotoxic liabilities in induced pluripotent stem cell-derived cardiomyocytes using a single-parameter score based on deep learning.

Mon Aug 02 00:00:00 UTC 2021

SK Channel Modulates Synaptic Plasticity by Tuning CaMKIIα/β Dynamics.

Frontiers in synaptic neuroscience

Shrestha A,Sultana R,Lee CC,Ogundele OM

701914 was used in Immunohistochemistry-immunofluorescence to demonstrate that positive modulation of SK channel at hippocampal synapses likely refine GluN1-linked plasticity by tuning dendritic localization of CaMKIIα.