Invitrogen

c-Jun Polyclonal Antibody

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c-Jun Antibody (PA1-833) in WB

Western Blot analysis of cJun in NIH 3T3 cells using Product # PA1-833 after serum stimulation.

c-Jun Antibody in Western Blot, ChIP Assay (WB, ChIP)

Product Image Gallery

c-Jun Antibody — Jun expression and contribution to ESC state. (A): Quantification of Jun mRNA levels after release from inhibition with 2i over a 5 day period. The values are normalized against Gapdh and are relative to day 0 (taken as 1). Error bars represent SEM (n = 3) (*, p < .05). (B): Western blot analysis of Jun protein expression in ESCs upon release from culturing in 2i. Samples were collected at day 0 (lane 1 -in 2i), days 1, 2, and 3 (lanes 2-4). The upper panel shows Jun protein expression, and the bottom panel was blotted with anti-Erk antibody as a loading control. Jun protein expression was quantified and normalized against Erk levels and is shown above the blots. The error bars represent the SEM (n = 3) (*, p < .05). The enrichment values compared to day 0 are indicated above the corresponding time points. (C): ESCs were treated by transient transfection with either NTC or JunsiRNA, released from the 2i inhibition and the expression of pluripotency and differentiation-associated genes was assessed at 0, 1, 3, and 5 days using the BioMark HD System (Fluidigm). The FC of the expression of each gene in the knockdown condition compared to the NTC condition are reported as a heatmap as log2(siRNA/NTC). For better visualization cut-offs of log2 = 1 and -1 were chosen. Red values represent increases and blue represents decreases in gene expression following Jun knockdown. The marker genes have been grouped together depending on their known role, which is indicated as different colors {KD}

产品信息

PA1-833

应用

建议稀释比

已发表文章

免疫印迹 (WB)

10 µg/mL

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染色质免疫沉淀实验 (ChIP)

1-3 µL

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues T(91) P T P T Q F L C P K N V T D(105) of mouse cJun.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

1 mg/mL

纯化类型

IgG fraction

保存液

PBS

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2129865

产品详细信息

PA1-833 detects cJun from mouse cells.

PA1-833 has been successfully used in Western blot and ChIP procedures. By Western blot, this antibody detects an ~40 kDa protein representing cJun in NIH 3T3 serum stimulated cells. A smaller band is also detected at ~26 kDa possibly representing a proteolytic fragment of cJun.

The PA1-833 immunogen is a synthetic peptide corresponding to residues T(91) P T P T Q F L C P K N V T D(105) of mouse cJun.

Reconstitute in 200 µL PBS to create a stock of 1 mg/mL.

靶标信息

c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Jun-Mediated Changes in Cell Adhesion Contribute to Mouse Embryonic Stem Cell Exit from Ground State Pluripotency.

Stem cells (Dayton, Ohio)

Veluscek G,Li Y,Yang SH,Sharrocks AD

PA1-833 was used in western blot to research mouse embryonic stem cell exit from ground state pluripotency by Jun-mediated changes in cell adhesion

Sun May 01 00:00:00 UTC 2016

Jun-Mediated Changes in Cell Adhesion Contribute to Mouse Embryonic Stem Cell Exit from Ground State Pluripotency.

Stem cells (Dayton, Ohio)

Veluscek G,Li Y,Yang SH,Sharrocks AD

PA1-833 was used in western blot to research mouse embryonic stem cell exit from ground state pluripotency by Jun-mediated changes in cell adhesion

Sun May 01 00:00:00 UTC 2016

生物信息学

蛋白别名: Activator protein 1; AH119; AP1; cjun; immediate early; Jun A; Jun oncogene; JUND; Proto-oncogene c-Jun; transcription factor AP-1; Transcription factor AP-1 subunit Jun; Transcription factor Jun; V-jun avian sarcoma virus 17 oncogene homolog

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基因别名: AP-1; c-jun; Jun; Junc

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UniProt ID: (Mouse) P05627

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Entrez Gene ID: (Mouse) 16476

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功能

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding DNA binding chromatin binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity GTPase activator activity protein binding transcription factor binding enzyme binding cAMP response element binding protein homodimerization activity sequence-specific DNA binding transcription regulatory region DNA binding poly(A) RNA binding protein heterodimerization activity R-SMAD binding HMG box domain binding

参与通路

c-Jun Polyclonal Antibody

c-Jun Antibody (PA1-833) in WB

产品信息

PA1-833

免疫印迹 (WB)

染色质免疫沉淀实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

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c-Jun Polyclonal Antibody

c-Jun Antibody (PA1-833) in WB

产品信息

PA1-833

免疫印迹 (WB)

染色质免疫沉淀 实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)