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eIF4H Antibody (PA5-115170) in ICC/IF
Immunocytochemistry analysis of eIF4H in A549 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115170). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG (Green) and DAPI.
产品信息
PA5-115170
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human EIF4H(Accession Q15056), corresponding to amino acid residues D8-I58.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2899806
产品详细信息
Antibody detects endogenous levels of total EIF4H.
靶标信息
This gene encodes one of the translation initiation factors, which functions to stimulate the initiation of protein synthesis at the level of mRNA utilization. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternative splicing of this gene generates 2 transcript variants.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: eIF-4H; Eukaryotic translation initiation factor 4H; Williams-Beuren syndrome chromosomal region 1 protein; Williams-Beuren syndrome chromosomal region 1 protein homolog; Williams-Beuren syndrome chromosome region 1; Williams-Beuren syndrome chromosome region 1 homolog
基因别名: AU018978; D5Ertd355e; E430026L18Rik; Ef4h; eIF-4H; EIF4H; KIAA0038; mKIAA0038; WBSCR1; WSCR1
UniProt ID: (Human) Q15056, (Mouse) Q9WUK2
Entrez Gene ID: (Human) 7458, (Mouse) 22384
nucleotide binding
RNA binding
translation initiation factor activity
helicase activity
protein binding
translation factor activity, RNA binding
RNA strand annealing activity
RNA strand-exchange activity
ribosomal small subunit binding
poly(A) RNA binding
cadherin binding involved in cell-cell adhesion
nucleic acid binding
translation initiation factor
