Search Thermo Fisher Scientific
产品规格
检测灵敏度
0.5 pg/mL
测定范围
1,6-100
检测样本类型/体积
{Serum=50 µL, Supernatant=50 µL, Plasma=50 µL}
总时间
3 hr 10 min
均相（无需洗涤）
No
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Coated Microwell Strips
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
Biotin-Conjugate
Streptavidin-HRP
Standard
Assay Buffer Concentrate
Wash Buffer Concentrate
Substrate Solution
Stop Solution
Adhesive Film
运输条件
Wet ice
储存
2-8°C
蛋白名称
IL-17A
蛋白家族
Hormones & Receptors
均相（无需洗涤）
Human
Species (reported)
Human
检测试剂盒格式
Basic ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human Interleukin-17A (Hu IL-17A) ELISA kit facilitates the accurate quantification of Hu IL-17A in various biological samples, including human serum, plasma, buffered solutions, or cell culture medium. This highly specific assay is designed to exclusively detect both endogenous and recombinant forms of Hu IL-17A.
原理方法
The Human IL-17A solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) utilizes a meticulously paired set of antibodies to capture and measure the target analyte. The microplate wells are pre-coated with a target-specific antibody, enabling the binding of the analyte during the assay. Following the addition of samples or standards to the wells, they bind to the immobilized (capture) antibody. The formation of a sandwich complex is achieved by introducing a second (detector) antibody. Subsequently, a substrate solution is added, initiating a reaction with the enzyme-antibody-target complex and generating a measurable signal. The intensity of this signal is directly proportional to the concentration of the target present in the original specimen.
靶标信息
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
基因别名 : CTLA-8, CTLA8, IL-17, IL-17A, IL17, IL17A
基因ID : (Human) 3605
基因符号 : IL17A
蛋白别名 : CTLA-8, Cytotoxic T-lymphocyte-associated antigen 8, cytotoxic T-lymphocyte-associated protein 8, IL-17, IL-17A, interleukin 17, R-IL-17-A, Interleukin17A, il 17, ILN, Interleukin, interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8), Interleukin-17A
UniProt ID (Human) Q16552
