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产品规格
检测灵敏度
0.1 n ng/mL
测定范围
0.16-10 ng/mL
检测样本类型/体积
{Serum=100 µL, Plasma=100 µL, Tissue Homogenate=100 µL, Cell Lysate=100 µL}
手工操作时间
1 hr 20 min
总时间
2 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
Ferritin
均相（无需洗涤）
Human
Species (reported)
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
Unconjugated
关于本试剂盒
The Human Ferritin (FE) ELISA quantitates FE in serum, plasma and other biological fluids.
原理方法
The Human Ferritin (FE) solid phase sandwich ELISA (enzyme linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target specific antibody has been pre coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
靶标信息
Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. Ferritin is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in the light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. The ferritin gene has multiple pseudogenes, and ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis. Ferritin is a holoenzyme shell with a molecular weight of about 450 kDa, and consists of 24 subunits of two types, H(heavy) and L(light), and capable of storing up to 4,500 atoms of ferric iron.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : FHC, FTH, FTH1, FTHL6, FTL, HFE5, LFTD, NBIA3, OK/SW-cl.84, PIG15, PLIF
基因ID : (Human) 2495, (Human) 2512
基因符号 : FTH1, FTL
蛋白别名 : apoferritin, Cell proliferation-inducing gene 15 protein, Ferritin H subunit, Ferritin heavy chain, N-terminally processed, Ferritin L subunit, ferritin L-chain, Ferritin light chain, ferritin light polypeptide-like 3, ferritin, heavy polypeptide 1, light polypeptide, L apoferritin, MGC71996, frih, fril, placenta immunoregulatory factor, proliferation-inducing protein 15
UniProt ID (Human) P02794, (Human) P02792
