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产品规格

检测灵敏度

<0.02 ng/mL

测定范围

0.0256-2000 ng/mL

检测样本类型/体积

{Serum=2-5 µL, Plasma=2-5 µL, Supernatant=2-5 µL}

手工操作时间

30 min

总时间

2 hr

均相（无需洗涤）

Yes

检测仪器

qPCR Instruments

产品规格

96 Tests

产品成分

Assay Dilution Buffer
Antibody-Conjugate A
Antibody-Conjugate B
Antibody-Conjugate Dilution Buffer
Standard
Master Mix
Ligase

运输条件

Dry ice

储存

-20°C

蛋白名称

Human IgA

均相（无需洗涤）

Human

检测试剂盒格式

Proximity-based Immunoassay

标签或染料

FAM™

关于本试剂盒

The ProQuantum Human IgA Immunoassay Kit is designed to provide quantitative measurements of human IgA in small sample volumes. Utilizing proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly workflow combined with intuitive software for analytics enables sample-to-answer in just 2 hours.

• High sensitivity-detect low levels of protein with greater sensitivity than traditional methods

• Broad dynamic range-5 logarithmic units, minimizing sample dilutions to ensure they fall within the range

• Small sample consumption-use 2-5 µL of sample (compared to 75 µL for triplicate wells with other methods)

• Fast, easy workflow-2 hours from sample to answer, with no wash steps

• No proprietary instrument to purchase-runs on any real-time PCR instrument

ProQuantum immunoassays utilize a matched pair of target-specific antibodies, each conjugated to a DNA oligonucleotide. During antibody-analyte binding, the two DNA oligos are brought into close proximity, which allows for ligation of the two strands and subsequent creation of a template strand for amplification. This platform leverages the sensitivity and large dynamic range of Applied Biosystems TaqMan real-time PCR technology (Figure 1).

The assay workflow is fast and easy-2 steps in 2 hours. There are a total of 7 components in each kit (Figure 2). First, mix the antibody-conjugates, dilute the sample, and create the standard curve in a working plate. Then, using a multi-channel pipette, add the antibody-conjugates and sample (or standard) into the wells of a PCR plate and incubate for 1 hour. Combine the master mix and ligase and add to the wells of the PCR plate, then run the plate on any qPCR instrument. After the run is complete, import the results file into the ProQuantum software (cloud-based version at https://apps.thermofisher.com/apps/proquantum). Using this software, the data can be analyzed easily to obtain protein concentration values. The software allows you to set up standard curves, design plate layouts, apply a 5PL weighted algorithm, and obtain robust statistical group-wise comparisons.

靶标信息

Human IgA, also known as human immunoglobulin A, is an antibody that is produced by the immune system in response to antigens. It is the most abundant immunoglobulin in mucosal secretions, such as saliva, tears, and breast milk, and plays a key role in defense against pathogens at mucosal surfaces. IgA works by binding to specific antigens and preventing them from entering the body's tissues. It can also activate complement and promote phagocytosis by immune cells. Human IgA can be used in research to study the immune response to pathogens and to develop diagnostic tests and therapeutic interventions for infectious diseases.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

基因别名 : IgA1

基因ID : (Human) 3493, (Human) 3494

基因符号 : IGHA1, IGHA2

蛋白别名 : Immunoglobulin A, Ig alpha 1 Chain C Region, Ig alpha 2 Chain C Region, Immunoglobulin Am1, Immunoglobulin Am2, Immunoglobulin Heavy Constant Alpha 2, A2m Marker

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