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产品规格
测定范围
15.6-1000 pg/ml
检测样本类型/体积
{Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 15 min
均相（无需洗涤）
No
批间差异
<8%
批内差异
<8%
检测仪器
Colorimetric Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well Microplate
Detection Antibody Concentrate
Anti-IgG-HRP Concentrate
Concentrate Diluent
Standard
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Adhesive Plate Covers
Detection Antibody Concentrate
Anti-IgG-HRP Concentrate
Concentrate Diluent
Standard
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
IFN beta
蛋白家族
Cytokines & Receptors
均相（无需洗涤）
Mouse
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Mouse Interferon beta (Ms IFNβ) ELISA quantitates Ms IFNβ in mouse cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IFNβ.
原理方法
The Mouse IFNβ solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by Natural IFN-producing cells (IPCs) as part of the host immune response. IFNs can also inhibit the development of tumors. IFN-beta binding results in the activation of the tyrosine kinases Jak1 and Tyk2, phosphorylation of members of the STAT family of transcription factors, and the transcription and expression of the immune response genes. More recently, several members of the toll-like receptor (TLR) family were found to stimulate the production IFN-beta. IFN-beta is currently used clinically for treatment of tumors, infections and multiple sclerosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : Ifb, IFN-beta, IFNB, Ifnb1
基因ID : (Mouse) 15977
基因符号 : Ifnb1
蛋白别名 : IFN-beta, RP11-113D19.1, IFN b, IFN β, IFNbeta, IFNβ, Interferon b, Interferon, Interferon beta
UniProt ID (Mouse) P01575
