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The Rat Ferritin ELISA quantitates Ferritin in serum, plasma, and other biological fluids
The Rat Ferritin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. Ferritin is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in the light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. The ferritin gene has multiple pseudogenes, and ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis. Ferritin is a holoenzyme shell with a molecular weight of about 450 kDa, and consists of 24 subunits of two types, H(heavy) and L(light), and capable of storing up to 4,500 atoms of ferric iron.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
基因别名 : Fth, Fth1, Ftl, Ftl1
基因ID : (Rat) 25319, (Rat) 29292
基因符号 : Fth1, Ftl1
蛋白别名 : Ferritin H subunit, Ferritin heavy chain, ferritin heavy chain 1, N-terminally processed, Ferritin L subunit 1, Ferritin light chain 1, Ferritin subunit H, ferritin, light polypeptide, L apoferritin, MGC71996, frih, fril
UniProt ID (Rat) P19132, (Rat) P02793