Each Quantifiler Duo kit includes all reagents necessary for the amplification, detection, and quantification of 400 samples in 25 ul reactions using the Applied Biosystems 7500 Real-Time PCR System.

Component Composition
Quantifiler Duo Primer MixPrimer pairs for amplification of RPPH1, SRY and IPC. The TaqMan probes for RPPH1, SRY and IPC are labeled with VIC, FAM™ and NED™ dyes, respectively. In addition, this reagent contains Internal PCR Control (IPC) template.
Quantifiler Duo PCR Reaction MixAmpliTaq Gold DNA Polymerase in buffer and salts
Quantifiler Duo DNA StandardPooled human male genomic DNA
Quantifiler Duo Dilution Buffer10 mM Tris HCl buffer, pH 8.0 containing 0.1 mM EDTA