Each Quantifiler Duo kit includes all reagents necessary for the amplification, detection, and quantification of 400 samples in 25 ul reactions using the Applied Biosystems 7500 Real-Time PCR System. Component Composition Quantifiler Duo Primer Mix Primer pairs for amplification of RPPH1, SRY and IPC. The TaqMan probes for RPPH1, SRY and IPC are labeled with VIC, FAM™ and NED™ dyes, respectively. In addition, this reagent contains Internal PCR Control (IPC) template. Quantifiler Duo PCR Reaction Mix AmpliTaq Gold DNA Polymerase in buffer and salts Quantifiler Duo DNA Standard Pooled human male genomic DNA Quantifiler Duo Dilution Buffer 10 mM Tris HCl buffer, pH 8.0 containing 0.1 mM EDTA