Increased amplicon sizes for improved prediction of STR amplification performance and estimation of DNA quantity in degraded samples
Similar amplicon sizes for all three targets to avoid preferential PCR
Single copy targets for superior accuracy and consistency in quantification results (multi-copy targets are affected by variability between individuals and decrease correlation with the single copy targets in STR kits)
Validated on the 7500 Real-Time PCR System using SDS v1.2.3 software