The Quantifiler Duo kit uses an alternative human DNA target (RPPH1) and increased amplicon sizes to improve prediction of STR amplification performance and estimation of DNA quantity in degraded samples
The Quantifiler Duo DNA Standard is pooled human male genomic DNA (in contrast to cell-line DNA) for improved stability and correlation with forensic samples
The Quantifiler Duo PCR Reaction Mix has been optimized for expanded multiplex performance
The Quantifiler Duo kit has been designed to guide selection of appropriate AmpFℓSTR Kit chemistry and maximize recovery of interpretable STR profiles
The dynamic range of the Quantifiler Duo kit is 23pg-50ng/µL (the same as previous Quantifiler kits), but the highly sensitive assay has consistently demonstrated the ability to detect samples with a DNA concentration as low as 6pg/µL. Please note, however, that detection of <100pg/µL using any amplification-based quantification method is subject to stochastic effects which may result in some uncertainty in quantification results and prediction of amplification success.