showcase.par.64038.image.0.0.1

实时检测激酶活性

  • 操作简便-只需混合,然后实时读取荧光值
  • 动力学模式 - 适用于力学研究
  • 底物抑制剂研究
  • 快速-可在一小时之内获取结果

Omnia Kinase Assays 激酶检测试剂盒利用荧光肽为反应底物,快速、均匀和灵敏地检测酶活性。在不使用放射性示踪剂或专用设备的情况下,在最佳动力学、最适生理浓度 ATP 下进行实时测量获得实验结果。荧光值随激酶活性的增加而相应增加。该检测的动力学模式还纠正了假阳性(化合物自动荧光)和假阴性(猝灭),最大限度地减少干扰。该方法可用于ATP竞争性抑制剂和ATP非竞争性抑制剂(变构)的筛选。

了解更多关于 Omnia 激酶原理 >

激酶(共计 112)推荐试剂盒
AKT1ST1
AKT2ST1
AKT3ST1
AMPKST23
ARK5ST31
AURORA AST16
AURORA BST16
AURORA CST16
BRSK1 
CAMK1D(CAMKIδ)ST23
CAMK2A(CAMKIIα)ST23
CAMK2D(CAMKIIδ)ST23
CDC42BPA(MRCK/DMPK)ST18
CDC42BPB(MRCK/DMPK)ST18
CDK1/CyclinBST7
CDK1/CyclinEST7
CDK2/CyclinAST7
CDK2/CyclinEST7
CDK4/CyclinD1 
CDK5/p25NCKST7
CDK5/p35ST7
CDK6/CyclinD1 
CHEK1(CHK1)ST11
CHEK2(CHK2)ST3
CLK2ST15
CLK3ST15
CSNK1A1(CK1)ST24
CSNK1D(CKIδ)ST24
CSNK1E(CK1ε)ST24
CSNK1G1(CK1γ1)ST24
CSNK1G2(CK1γ2)ST24
CSNK1G3(CK1γ3)ST24
CSNK2A1(CK2α1)ST22
DAPK3ST1
DCAMKL2 
DYRK1A 
GSK3αST13
GSK3β(C 端-组氨酸)ST13
IKKβ(IKK2)ST20
IKKεST24
MAP3K10(MLK2)ST6
MAP3K11(MLK3)ST8
MAP3K9(MLK1)ST8
MAP4K2(GCK)ST25
MAP4K4(HGK)ST32
MAP4K5(KHS1)ST26
MAPK1(ERK2)ST17
MAPK10(JNK3)ST10
MAPK11(p38β)ST9
MAPK12(p38γ)ST7
MAPK13(p38δ) 
MAPK14(p38α) 
MAPK3(ERK1)ST17
MAPK8(JNK1)ST10
MAPKAP-K2(MK2)ST3
MAPKAP-K3ST3
MAPKAP-K5(PRAK)ST3
MARK2ST31
MINK1ST8
MLK1ST8
MLK3ST8
NEK1 
NEK2 
NEK4 
NEK6ST27
NEK9 
PAK1ST5
PAK2ST5
PAK3ST5
PAK4ST5
PAK6ST5
PAK7ST5
PASKST6
PHKG1ST3
PHKG2ST3
PIM1ST26
PIM2ST14
PKAST5
PKCαST8
PKCβIST8
PKCβIIST8
PKCδST8
PKCεST8
PKCηST8
PKCγST8
PKCιST8
PKCθST8
PKCζST8
PKCμST8
PKN1(PRK1)ST18
PRKCN(PKD3)ST8
PRKD2ST8
PRKG1ST5
PRKG2ST5
PRKXST5
ROCK1ST5
ROCK2ST5
RPS6KA1(RSK1)ST6
RPS6KA2(RSK3)ST6
RPS6KA3(RSK2)ST6
RPS6KA5(MSK1)ST1
RPS6KA6(RSK4)ST6
RPS6KB1(p70S6K)ST6
SGK(SGK1)ST1
SGK2ST4
SGKL(SGK3)ST4
STK22B(TSSK2)ST31
STK22D(TSSK1) 
STK3(MST2) 
STK4(MST1) 
TAOK2(TAO1) 
TBK1(IKK-ε)ST12

 

激酶(共 77 个)最佳试剂盒
ABL1Y6
ABL1 E255KY6
ABL1 G250EY6
ABL1 T315IY6
ABL1 Y253FY6
ABLl2(ARG)Y6
ALKY4
AXLY11
BLKY5
BMXY5
BTKY5
CSF-1R(FMS)Y9
EGFR(ErbB1)Y12
EGFR T790MY12
EGFR L858RY10
EGFR L861QY12
EGFR1Y7
EPHA1Y6
EPHA2Y5
EPHA4Y12
EPHA5 
EPHA8Y12
EPHB1Y6
EPHB2Y6
EPHB3Y6
EPHB4Y5
FERY5
FGFR1Y12
FGFR2Y11
FGFR3Y11
FGFR3 K650EY12
FGFR4Y10
FGRY5
FLT1(VEGFR1)Y8
FLT3 CDY5
FLT3 D835YY5
FLT4(VEGFR3)Y8
FRKY5
FYNY5
HCKY5
IGF-1RY4
IR(胰岛素受体)Y4
IRR(胰岛素相关受体)Y4
ITKY6
JAK2Y11
JAK2 JH1 JH2Y12
JAK2 JH1 JH2 V617FY12
JAK3Y7
KDR(VEGFR2)Y8
KITY9
KIT T670IY12
LCKY5
LYN AY5
LYN BY5
MERTK(cMER)Y6
MET(HGFR)Y12
MET(HGFR)M1250TY9
MST1RY8
MUSKY1
NTRK1(TRKA)Y8
NTRK2(TRKB)Y5
PDGFRA(PDGFRα)Y9
PDGFRA D824VY9
PDGFRB(PDGFRβ)Y14
PTK2(FAK)Y3
RETY12
RET V804LY8
RET Y791FY11
ROS1Y4
SrcY2
SRCN2 
SRMSY12
SYKY7
TEK(tie2)Y8
TYRO3(RSE)Y5
Y5
ZAP70Y8
激酶(共计 112)推荐试剂盒
AKT1ST1
AKT2ST1
AKT3ST1
AMPKST23
ARK5ST31
AURORA AST16
AURORA BST16
AURORA CST16
BRSK1 
CAMK1D(CAMKIδ)ST23
CAMK2A(CAMKIIα)ST23
CAMK2D(CAMKIIδ)ST23
CDC42BPA(MRCK/DMPK)ST18
CDC42BPB(MRCK/DMPK)ST18
CDK1/CyclinBST7
CDK1/CyclinEST7
CDK2/CyclinAST7
CDK2/CyclinEST7
CDK4/CyclinD1 
CDK5/p25NCKST7
CDK5/p35ST7
CDK6/CyclinD1 
CHEK1(CHK1)ST11
CHEK2(CHK2)ST3
CLK2ST15
CLK3ST15
CSNK1A1(CK1)ST24
CSNK1D(CKIδ)ST24
CSNK1E(CK1ε)ST24
CSNK1G1(CK1γ1)ST24
CSNK1G2(CK1γ2)ST24
CSNK1G3(CK1γ3)ST24
CSNK2A1(CK2α1)ST22
DAPK3ST1
DCAMKL2 
DYRK1A 
GSK3αST13
GSK3β(C 端-组氨酸)ST13
IKKβ(IKK2)ST20
IKKεST24
MAP3K10(MLK2)ST6
MAP3K11(MLK3)ST8
MAP3K9(MLK1)ST8
MAP4K2(GCK)ST25
MAP4K4(HGK)ST32
MAP4K5(KHS1)ST26
MAPK1(ERK2)ST17
MAPK10(JNK3)ST10
MAPK11(p38β)ST9
MAPK12(p38γ)ST7
MAPK13(p38δ) 
MAPK14(p38α) 
MAPK3(ERK1)ST17
MAPK8(JNK1)ST10
MAPKAP-K2(MK2)ST3
MAPKAP-K3ST3
MAPKAP-K5(PRAK)ST3
MARK2ST31
MINK1ST8
MLK1ST8
MLK3ST8
NEK1 
NEK2 
NEK4 
NEK6ST27
NEK9 
PAK1ST5
PAK2ST5
PAK3ST5
PAK4ST5
PAK6ST5
PAK7ST5
PASKST6
PHKG1ST3
PHKG2ST3
PIM1ST26
PIM2ST14
PKAST5
PKCαST8
PKCβIST8
PKCβIIST8
PKCδST8
PKCεST8
PKCηST8
PKCγST8
PKCιST8
PKCθST8
PKCζST8
PKCμST8
PKN1(PRK1)ST18
PRKCN(PKD3)ST8
PRKD2ST8
PRKG1ST5
PRKG2ST5
PRKXST5
ROCK1ST5
ROCK2ST5
RPS6KA1(RSK1)ST6
RPS6KA2(RSK3)ST6
RPS6KA3(RSK2)ST6
RPS6KA5(MSK1)ST1
RPS6KA6(RSK4)ST6
RPS6KB1(p70S6K)ST6
SGK(SGK1)ST1
SGK2ST4
SGKL(SGK3)ST4
STK22B(TSSK2)ST31
STK22D(TSSK1) 
STK3(MST2) 
STK4(MST1) 
TAOK2(TAO1) 
TBK1(IKK-ε)ST12

 

激酶(共 77 个)最佳试剂盒
ABL1Y6
ABL1 E255KY6
ABL1 G250EY6
ABL1 T315IY6
ABL1 Y253FY6
ABLl2(ARG)Y6
ALKY4
AXLY11
BLKY5
BMXY5
BTKY5
CSF-1R(FMS)Y9
EGFR(ErbB1)Y12
EGFR T790MY12
EGFR L858RY10
EGFR L861QY12
EGFR1Y7
EPHA1Y6
EPHA2Y5
EPHA4Y12
EPHA5 
EPHA8Y12
EPHB1Y6
EPHB2Y6
EPHB3Y6
EPHB4Y5
FERY5
FGFR1Y12
FGFR2Y11
FGFR3Y11
FGFR3 K650EY12
FGFR4Y10
FGRY5
FLT1(VEGFR1)Y8
FLT3 CDY5
FLT3 D835YY5
FLT4(VEGFR3)Y8
FRKY5
FYNY5
HCKY5
IGF-1RY4
IR(胰岛素受体)Y4
IRR(胰岛素相关受体)Y4
ITKY6
JAK2Y11
JAK2 JH1 JH2Y12
JAK2 JH1 JH2 V617FY12
JAK3Y7
KDR(VEGFR2)Y8
KITY9
KIT T670IY12
LCKY5
LYN AY5
LYN BY5
MERTK(cMER)Y6
MET(HGFR)Y12
MET(HGFR)M1250TY9
MST1RY8
MUSKY1
NTRK1(TRKA)Y8
NTRK2(TRKB)Y5
PDGFRA(PDGFRα)Y9
PDGFRA D824VY9
PDGFRB(PDGFRβ)Y14
PTK2(FAK)Y3
RETY12
RET V804LY8
RET Y791FY11
ROS1Y4
SrcY2
SRCN2 
SRMSY12
SYKY7
TEK(tie2)Y8
TYRO3(RSE)Y5
Y5
ZAP70Y8

表 1.试剂订购信息

试剂货号
Omnia Ser/Thr 反应缓冲液KB001A
Omnia Tyr 反应缓冲液KB002A
Omnia ATP 溶液AS001A

Omnia 定制和批量衬底服务

如果您没有发现您感兴趣的底物,Invitrogen 还提供定制 Omnia 肽底物的服务。Omnia 底物可以成功地用于多种激酶。若您正在进行大型筛选项目,我们可提供毫克数量的冻干基质。

发邮件至discoverysciences@invitrogen.com,了解更多服务信息或询价。