photo showing Qubit® 2.0 Fluorometer with USB drive inserted 

IMPORTANT NOTE: The method for updating the firmware of your particular Qubit 2.0 Fluorometer is dependent upon two variable factors:

  • The numeric range containing the serial number of your Qubit 2.0 Fluorometer AND
  • The computer platform you are using to upgrade your Qubit 2.0 Fluorometer: Windows or Mac

Qubit 2.0 firmware update

For Qubit 2.0 Fluorometers with serial numbers below 1104004846, please use a Windows operating system to update your Qubit 2.0 Fluorometer using the instructions on this page. If you do not have access to a PC, please contact Technical Support for further instructions. Firmware upgrades for Qubit 2.0 Fluorometers with serial numbers 1104004846 and higher, are compatible with both Windows and Mac operating systems using the instructions on this page.

Use Table 1 to determine if you can use the instructions detailed on this page to update the firmware of your Qubit 2.0 Fluorometer.

Table 1. How To update the firmware for your Qubit 2.0 Fluorometer.

Qubit 2.0 serial number
Operating system
 Windows Mac
Below 1104004846Use instructions below
Contact Technical Support
1104004846 and above
Use instructions belowUse instructions below

Step-by-step firmware update instructions (if applicable)

1. Click on “Download the Latest Firmware Ver 3.11” (This will be the .zip file)

download the latest firmware v3.11


2. Click on the “Open” button from the File Download pop-up window

PC dialog box showing how to open the firmware file


3. Click on “Extract all files"

PC screenshot showing how to extract files


4. From the Extraction Wizard, click “Next“

PC screenshot showing how to click next in the extraction wizard


5. Select a folder to extract the files to; click “Next“



6. Click “Finish”

PC dialog box showing clicking finish button


7. Highlight the two .bin files, V3.11 Qubit_FW_MainCPU.bin and V3.10 Qubit_FW_UsbHost.bin, right-click using your mouse and select “Copy”

PC screenshot showing how to copy two .bin files


8. Insert a USB drive into your PC and search for this drive by clicking “My Computer”

PC screenshot showing how to find USB drive inserted into the PC


9. Right-click on your USB drive (Here, drive “E:”) and select “Paste” from the drop-down menu

PC screenshot showing how to paste copied .bin files to USB drive


10. Update Files Are Ready

The Qubit 2.0 firmware upgrade files are now on your USB drive and your instrument is ready to be updated. You can check by double-clicking on the USB drive. Make sure your USB drive contains both Qubit .bin files. These 2 files must both be present on the USB drive for the upgrade to be successful.

PC screenshot showing .bin files correctly copied to USB drive


11. On your Qubit 2.0 Fluorometer, press the Version button located on the top portion of the touch screen; the Version Screen is displayed

Qubit instrument screenshot showing version screen


12. Insert the USB drive into the USB port and press Update.

After the successful update of the firmware, the following screen is displayed before the instrument returns to the Home Screen. You can now proceed to setting your standards and quantitating your samples.

Qubit instrument screenshot showing firmware update complete message Note: A green dot on the Update button indicates that the instrument recognizes the USB drive; a red dot indicates that the USB drive is not inserted into the USB port or that the instrument does not recognize the USB drive.