Absorbance- and fluorescence-based nucleic acid quantification methods each have their own advantages. With the advances of UV-Vis software, researchers can now selectively quantify nucleic acid absorbance in the presence of chemical and nucleic acid contaminants. Regardless of your technology needs, Thermo Scientific NanoDrop instruments will provide you quick and accurate results.
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|How does it work?||Absorbance of UV-Visible light||Fluorescence|
|What is the usable concentration range?||0.2* – 27,500 ng/µL|
*using 10 mm cuvette
|Can it detect contaminants?||Yes; Thermo Scientific Acclaro Sample Intelligence technology can detect several contaminants in DNA, RNA and protein samples.||No: quantification only|
|Can I selectively measure dsDNA/ssDNA/RNA even when the others are present?||Coming in October 2020: In some applications, Acclaro algorithms are able to detect dsDNA contamination in RNA and RNA contamination in dsDNA.||Yes: assays are selective for dsDNA/ssDNA/RNA|
|Which is faster?||No specific sample preparation needed. Measurement takes less than 8 seconds.||Requires reagent preparation and mixing with samples and measurement of standards.|
|Do I need a standard curve?||No||Yes|
|Is 21CRF Part 11 software available?||Yes||No|
NanoDrop products use a patented sample retention technology that allow UV-Vis measurements to be made from 1µL of sample with no cuvettes or dilutions. The NanoDrop family of products began with the popular NanoDrop 1000 Spectrophotometer, which made absorbance measurements fast and easy. The NanoDrop One and NanoDrop OneC Spectrophotometers are the latest generation micro-volume UV-Vis spectrophotometers continuing the legacy of simple and accurate quantitation. The NanoDrop 8000 UV-Vis Spectrophotometer uses the same technology but also allows scientists to take absorbance measurements of up to 8 samples at one time. Visit the NanoDrop Products Guide for more information about UV-Vis instruments.
NanoDrop products combined extensive expertise in micro-volume instrumentation and understanding of fluorescence applications to develop an innovative fluorospectrometer. The NanoDrop 3300 Fluorospectrometer utilizes a patented sample retention system to deliver fast and easy fluorescence measurements.
All NanoDrop instruments utilize a patented* sample retention system that allows the quantification of DNA and RNA from 1–2µL samples. *Patents US6628382 and US6809826.