One-Step reagents are used to directly amplify RNA samples on your real-time PCR instrument. The reverse transcription (RT) and qPCR steps are both conducted in the same reaction well. This reduces the number of sample manipulations required and time to result, but unlike two-step strategies, all of the cDNA is used in the PCR, so a cDNA archive is not available. One-step RT-PCR is most commonly used to process many samples at a time, for one or a few targets per sample. Examples include virus detection and quantification, high-throughput gene expression screening, and simple RNA quantification experiments.
Which One-Step Real-Time RT-PCR Master Mix Is Right for You?
| Order now | Order now | |
| Standard mode | Fast mode | |
|---|---|---|
| RNA-to-Ct 1-Step Kit | TaqMan Fast Virus 1-Step Master Mix | |
| Applications | RNA viral detection/quantitation DNA viral/pathogen detection/quantitation Gene Expression Multiplexing | RNA viral detection/quantitation DNA viral/pathogen detection/quantitation Gene Expression Multiplexing |
| Detection Chemistry | TaqMan SYBR | TaqMan |
| Sensitivity | High | High |
| Cycling/Run Time | 120 minutes | <60 min |
| Tolerant to PCR Inhibitors | ✓ | |
| Reverse Transcriptase | Array Script UP | Thermostable MMLV |
| ROX Reference Dye to Normalize Signal & Ensure Data Integrity | ✓ | ✓ |
| Format | 2 Tubes TaqMan RT-PCR Mix (2x) and TaqMan RT Enzyme Mix (40x) | 1 Tube (4X Mix) |
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