Invitrogen

Human Aquaporin 12 (aa 246-290) Control Fragment Recombinant Protein

说明书

技术支持

说明书

技术支持

产品信息

RP-104335

应用

建议稀释比

已发表文章

对照试剂 (Ctrl)

Assay-dependent

阻断反应 (BLOCK)

Assay-dependent

产品规格

种属

Human

Expression System

E. coli

氨基酸序列

FQRNLFYGQKNKYRAPRGKPAPASGDTQTPAKGSSVREPGRSGVE

分类

Recombinant

类型

Protein

纯度

>80% by SDS-PAGE and Coomassie blue staining

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

≥5.0 mg/mL

纯化类型

purified

保存液

PBS/1M urea, pH 7.4

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

产品详细信息

Highest antigen sequence indentity to the following orthologs: Mouse (42%), Rat (42%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-59918. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

靶标信息

Aquaporin 12 (AQP12) is the most recently identified member of the mammalian AQP family and is specifically expressed in pancreatic acinar cells. In vitro expression studies have revealed that AQP12 is localized at intracellular sites. To determine the physiological roles of AQP12 in the pancreas, we generated knockout mice for this gene (AQP12-KO). No obvious differences were observed under normal conditions between wild-type (WT) and AQP12-KO mice in terms of growth, blood chemistry, pancreatic fluid content, or histology. However, when we induced pancreatitis through the administration of a cholecystokinin-8 (CCK-8) analog, the AQP12-KO mice showed more severe pathological damage to this organ than WT mice.

仅用于科研。不用于诊断过程。未经明确授权不得转售。